We narrowed to 1,791 results for: plasmid for e coli
-
Plasmid#240162PurposePlasmid encoding Oropouche virus (OROV) nucleoprotein (N) with an N-terminal His6 and biotin acceptor peptide (BAP, a.k.a. AviTag) for expression in E. coliDepositorInsertNucleoprotein
Tags6xHis, Biotin acceptor peptide (BAP, a.k.a. AviTa…ExpressionBacterialAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-NBK-1
Plasmid#207639PurposeA plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.DepositorInsertPyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
ExpressionBacterialMutationThe evolved synthetase NBK-1 contains mutations Y…PromoteraraBADAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)
Plasmid#191650PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletionDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MERS-coV-NSP3-c001
Plasmid#173085PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
TagsmCeruleanExpressionBacterialMutationD1109-D1275, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAIDA_TEV_fap2EC
Plasmid#246866PurposeExpression of Fusobacterium nucleatum ATCC23726 Fap2 extracellular domain (42-3271) with histag and TEV site in fusion with E. coli autotransporter AIDADepositorInsertF. nucleatum ATCC23726 Fap2 (extracellular domain, res. 42–3271)
Tags8x histidine, AIDA autotransporter, AIDA signal s…ExpressionBacterialAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)
Plasmid#191651PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4DepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-optimized E6AP F727D
Plasmid#165101Purposeecoli optimized expression of a GST fusion of Human E6AP F727DDepositorInsertE6AP F727D (UBE3A Human)
TagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE
Plasmid#234453PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4s-NGR
UseAAV; Flp/frtTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterSynapsinAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE
Plasmid#234454PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4f-NGR
UseAAV; Flp/frtTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterSynapsinAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-351_Cacnes_dCas9_CRISPRi
Plasmid#225617PurposeCutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
hSRAP.TWIN1.a13-87
Plasmid#49816Purposeexpression plasmid for SRAP(13-87) in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 13-87 (SRA1 Human)
Tagsintein & chitin binding domainExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pD441-H6-SUMO-HyPerFLEX
Plasmid#216676PurposePlasmid with cleavable His-tag for expression of the HyPer-FLEX sensor for hydrogen peroxide in E. coliDepositorInsertHyPerFLEX
Tags6xHis-SUMOExpressionBacterialPromoterT5Available SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIS_TEV_hsSYX3
Plasmid#179290PurposeE. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage siteDepositorInsertSyx
TagsHis6 + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7lacAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H11
Plasmid#184277PurposeExpresses nanobody H11 in E. coliDepositorInsertNanobody H11
TagsHis6ExpressionBacterial, Insect, and Mamm…PromoterT7Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINOH11-A10
Plasmid#184278PurposeExpresses nanobody H11-A10 in E. coliDepositorInsertNanobody H11-A10
TagsHis6ExpressionBacterial, Insect, and Mamm…PromoterT7Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H11-B5
Plasmid#184279PurposeExpresses nanobody H11-B5 in E. coliDepositorInsertNanobody H11-B5
TagsHis6ExpressionBacterial, Insect, and Mamm…PromoterT7Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_11-H6
Plasmid#184280PurposeExpresses nanobody H11-H6 in E. coliDepositorInsertNanobody H11-H6
TagsHis6ExpressionBacterial, Insect, and Mamm…PromoterT7Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINH11-H4 Q98R_H100E
Plasmid#184281PurposeExpresses nanobody H11-H4 Q98R_H100E in E. coliDepositorInsertNanobody H11-H4 Q98R_H100E
TagsHis6ExpressionBacterial, Insect, and Mamm…PromoterT7Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pExp-His-zBasic-RBD-Avi
Plasmid#195000PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tagDepositorAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only