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pET-SOMA-T679A

Plasmid

#118936

Purpose

Used to express the T679A mutant form of the SOMA FRET sensor in E. coli.

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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gamma2 UTR shRNA (shRNA4)

Plasmid

#120796

Purpose

shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

Depositor

Angel de Blas

Article

Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

Insert

GABRG2 shRNA (Gabrg2 Rat)

Use

RNAi

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTW087

Plasmid

#115935

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 EBFP2

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW120

Plasmid

#115947

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 TetR SapI/BsaI Mut

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW085

Plasmid

#115933

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 mVenus

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107321

Purpose

Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1333S) is fused to SaCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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C-term png in pGEX4

Plasmid

#112975

Purpose

For protein expression and purification of the C-term of Drosophila png

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png (png Fly)

Expression

Bacterial

Mutation

wild-type C terminus

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 png genomic in Casper

Plasmid

#112971

Purpose

P element vector for transposon insertion of png genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png genomic DNA (png Fly)

Use

P insertion vector

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 M2 png-myc in Casper4

Plasmid

#112968

Purpose

P element vector for transposon insertion of Myc-tagged png into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png-myc (png Fly)

Use

P insertion vector

Tags

myc epitope

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a - Ec-ClpX D144L

Plasmid

#111514

Purpose

Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Tags

His tag

Expression

Bacterial

Mutation

Aspartatic acid 144 to Leucine

Promoter

T7

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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