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pCMV-gLuc-DR_pCMV-RedFireFly.gb

Plasmid

#242435

Purpose

Gaussia Luciferse with a Direct Repeat sequence in the 3’UTR recognised by the CasRx under the control of the CMV promoter, and a RedFirefly Luciferase driven by a CMV promoter.

Depositor

Diego Di Bernardo

Article

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

Gaussia Luciferase & Red Firefly Luciferase

Use

CRISPR and Luciferase

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-TO-CasRx(NLS)-T2A-mCherry

Plasmid

#242433

Purpose

CasRx (NLS-RfxCas13d-NLS) for RNA targeting under the CMV-TO promoter (CMV with two Tet-Operators)

Depositor

Diego Di Bernardo

Article

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

CasRx

Use

CRISPR

Tags

Tag / Fusion Protein: T2A mCherry

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisTdT

Plasmid

#216674

Purpose

Expresses his-tagged N-terminus truncated TdT in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

terminal deoxynucleotidyl transferase

Tags

his-tag

Expression

Bacterial

Mutation

1-138 truncation

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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HisH2A-InteinCBD-H2B

Plasmid

#240442

Purpose

Expresses H2A-Intein-Chitinbinding domain bound to H2B

Depositor

Mark Glover

Article

Panigrahi et al Nucleic Acids Res. 2025 Oct 14;53(19):gk

Inserts

HisH2A-Intein-CBD
H2B (H2B )

Tags

C-terminal fusion with intein-chitin binding doma…

Expression

Bacterial

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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p15GFPkv

Plasmid

#239039

Purpose

Expresses a + positively charged GFP variant (+15GFPKv) in mammalian cells

Depositor

Felipe Quiroz

Article

Avecilla et al ACS Sens. 2025 Mar 28;10(3):1857-1869. d

Insert

p15GFPKv-NES

Expression

Mammalian

Mutation

All surface-exposed Arg residues changed to Lysine

Promoter

CMV

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-Donor_CBh

Plasmid

#241376

Purpose

Bxb1-GA donor plasmid for cloning of payloads <20 kb with CBh promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).cpSFGFP.mIRFP670nano3

Plasmid

#244918

Purpose

cpSFGFP control with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mIRFP670nano3

Use

AAV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1829 OpIE2-Bn

Plasmid

#242438

Purpose

Insect OpIE2 promoter expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Insert

Barnase (J5X95_RS06160 Bacillus amyloliquefaciens)

Expression

Insect

Promoter

OpIE2

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1767 PUb-Bs-TRE-Bn

Plasmid

#242441

Purpose

Ae aegypti PUb promoter expressing barstar, and TRE expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Inserts

Barstar
DsRED
barnase

Expression

Insect

Promoter

AePUb, Hr5/IE1, and TRE

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2197_pPV2-ZF10-NS3-p65

Plasmid

#239700

Purpose

BsaI Golden Gate assembly plasmid encoding the SynZiFTR ZF10-NS3-p65 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

ZF10-NS3-p65

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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