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We narrowed to 9,269 results for: ef1

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Showing: 3441 - 3480 of 9269 results

  1. lenti-UCOE-dCas9-BFP-VP64

    Plasmid
    #196717
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196717-plasmid-map-sequence-id-388038

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  2. Gal4-TEAD4

    Plasmid
    #24640
    Depositor
    Kunliang Guan
    Insert
    TEAD-4 (TEAD4 Human)
    Tags
    Gal4
    Expression
    Mammalian
    Available Since
    May 13, 2010
    Availability
    Academic Institutions and Nonprofits only

  3. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

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  4. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  5. pLV Tor1a-Myc

    Plasmid
    #175134
    Purpose
    Lentiviral expression of Myc-tagged mouse Tor1a
    Depositor
    Larry Gerace
    Insert
    Tor1a (Tor1a Mouse)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175134-plasmid-map-sequence-id-352021

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  6. pLV V5-Senp2

    Plasmid
    #175118
    Purpose
    Lentiviral expression of V5-tagged mouse Senp2
    Depositor
    Larry Gerace
    Insert
    Senp2 (Senp2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175118-plasmid-map-sequence-id-351807

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  7. pCAS9id_TRP

    Plasmid
    #141254
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141254-plasmid-map-sequence-id-308894

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  8. pSBBi-RP CCS1

    Plasmid
    #139162
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    caffeine synthase 1 [Coffea arabica]
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139162-plasmid-map-sequence-id-309352

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  9. pSBBi-GB CaXMT1

    Plasmid
    #139168
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    7-methylxanthosine synthase 1 [Coffea arabica]
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139168-plasmid-map-sequence-id-309358

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  10. pLV-Puro_hCOL4A4-3FLAG

    Plasmid
    #198101
    Purpose
    lentiviral expression of hCOL4A4 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A4 (COL4A4 Human)
    Use
    Lentiviral
    Tags
    3FLAG
    Promoter
    EF1a
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    198101-plasmid-map-sequence-id-389065

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  11. pLV-Hygro_hCOL4A3

    Plasmid
    #198098
    Purpose
    lentiviral expression of hCOL4A3 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A3 (COL4A3 Human)
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    198098-plasmid-map-sequence-id-467424

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  12. pSBBi-Pur GFP-TM

    Plasmid
    #139185
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.
    Depositor
    Neal Devaraj
    Insert
    GFP-TM
    Use
    Transposon
    Expression
    Mammalian
    Mutation
    fused to membrane anchor
    Promoter
    EF1α
    Available Since
    March 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    139185-plasmid-map-sequence-id-309371

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  14. pAAV-double floxed-eNpHR-EYFP-WPRE-pA

    Plasmid
    #20949
    Purpose
    Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    halorhodopsin
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    20949-plasmid-map-sequence-id-200280

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  15. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

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  16. pSBBi-Pur LaG17-synNotch-TetRVP64

    Plasmid
    #158133
    Purpose
    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
    Depositor
    Neal Devaraj
    Insert
    LaG17-synNotch-TetRVP64
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    158133-plasmid-map-sequence-id-309383

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  17. pSBBi-Pur LaG17-synNotch-Gal4VP64

    Plasmid
    #158135
    Purpose
    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
    Depositor
    Neal Devaraj
    Insert
    LaG17-synNotch-Gal4VP64
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Available Since
    Oct. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158135-plasmid-map-sequence-id-309380

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  18. pLV V5-lamin B2

    Plasmid
    #175109
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb2
    Depositor
    Larry Gerace
    Insert
    Lmnb2 (Lmnb2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175109-plasmid-map-sequence-id-351741

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  19. CMV-AausFP1-Z1

    Plasmid
    #135048
    Purpose
    AausFP1 - bright green fluorescent protein expression from A. australis under CMV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    AausFP1
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV Promoter
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135048-plasmid-map-sequence-id-269407

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  20. ELL3 Streptag

    Plasmid
    #127282
    Purpose
    Expresses mouse ELL3 with streptactin tag; does it degrade Myc?
    Depositor
    Christine Milcarek
    Insert
    ELL3 (NM_145973) (ELL3 Mouse)
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127282-plasmid-map-sequence-id-250028

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  22. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid
    #125199
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125199-plasmid-map-sequence-id-243587

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  23. dCas9-ryt

    Plasmid
    #246022
    Purpose
    Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lctra, yellow, and RFPex
    Depositor
    Max Scott
    Inserts
    dCas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available Since
    Dec. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    246022-plasmid-map-sequence-id-492666

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  24. dCas9-tra135

    Plasmid
    #246023
    Purpose
    Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene
    Depositor
    Max Scott
    Inserts
    dCas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available Since
    Dec. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    246023-plasmid-map-sequence-id-492668

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  25. dCas9-tra246

    Plasmid
    #246024
    Purpose
    Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene
    Depositor
    Max Scott
    Inserts
    dCas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available Since
    Dec. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    246024-plasmid-map-sequence-id-492667

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  26. Cas9-tra246

    Plasmid
    #246020
    Purpose
    Expresses Cas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene
    Depositor
    Max Scott
    Inserts
    Cas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246020-plasmid-map-sequence-id-494616

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  27. Cas9-tra135

    Plasmid
    #246019
    Purpose
    Expresses Cas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene
    Depositor
    Max Scott
    Inserts
    Cas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246019-plasmid-map-sequence-id-494614

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  28. pSBbi-Pur-LPAR

    Plasmid
    #125205
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide
    Depositor
    Eric Davis
    Insert
    Lyn-Phospho-Activity-Reporter, Empty
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125205-plasmid-map-sequence-id-243607

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  29. pSBBi-RP TCS1

    Plasmid
    #139161
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Caffeine Synthase 1 [Camellia sinensis]
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139161-plasmid-map-sequence-id-309127

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  30. pSBBi-RP CaDXMT

    Plasmid
    #139165
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffee arabica dimethylxanthine methyl transferase (CaDXMT) ; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    3,7-dimethylxanthine N-methyltransferase 1 [Coffea arabica]
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139165-plasmid-map-sequence-id-309355

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  31. pSBBi-RP CsAncCS

    Plasmid
    #139167
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Camellia sinensis ancestral caffeine synthase (CsAncCS); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Predicted Camellia sinensis Ancestral Caffeine Synthase
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139167-plasmid-map-sequence-id-309357

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  32. pSBBi-RP CaMXMT

    Plasmid
    #139172
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase (CaMXMT); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    monomethylxanthine methyltransferase 1 [Coffea arabica]
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139172-plasmid-map-sequence-id-309362

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  33. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196712-plasmid-map-sequence-id-388051

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  34. pLV-Blast_hCOL4A5

    Plasmid
    #198100
    Purpose
    lentiviral expression of hCOL4A5 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198100-plasmid-map-sequence-id-389263

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  35. TK4-NLS-EGFP

    Plasmid
    #239046
    Purpose
    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation
    Depositor
    Marius Wernig
    Inserts
    EGFP
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    T2A-mycNLS-mTagBFP2 and c-myc-NLS
    Expression
    Mammalian
    Promoter
    CAG and EF1a
    Available Since
    May 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    239046-plasmid-map-sequence-id-473412

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  36. pLV V5-lamin B1

    Plasmid
    #175108
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb1
    Depositor
    Larry Gerace
    Insert
    Lmnb1 (Lmnb1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175108-plasmid-map-sequence-id-351795

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  37. pLV V5-Sun2

    Plasmid
    #175114
    Purpose
    Lentiviral expression of V5-tagged mouse Sun2
    Depositor
    Larry Gerace
    Insert
    Sun2 (Sun2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175114-plasmid-map-sequence-id-351772

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  39. pLV Erh-V5

    Plasmid
    #175123
    Purpose
    Lentiviral expression of mouse Erh-V5
    Depositor
    Larry Gerace
    Insert
    Erh (Erh Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175123-plasmid-map-sequence-id-351935

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  40. pLV-Blast_hCOL4A5-HA

    Plasmid
    #198103
    Purpose
    lentiviral expression of hCOL4A5 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Tags
    HA
    Promoter
    EF1a
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198103-plasmid-map-sequence-id-389259

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Showing: 3441 - 3480 of 9269 results