We narrowed to 11,255 results for: NSI;

1. pCSP1-mp53AS-6xMUT

   Plasmid

   #20904

   Depositor

   Stefano Piccolo

   Article

   Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

   Insert

   p53AS (Trp53 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   point mutation (Ser/Thr-Ala) of the 6 N-terminal …

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pCDF_AtRaf1/Raf2/BSD2

   Plasmid

   #229511

   Purpose

   Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,BSD2

   Depositor

   Laura Gunn

   Article

   Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

   Inserts

   Rubisco accumulation factor 1 (AT3G04550 Mustard Weed)
   Rubisco accumulation factor 2 (AT5G51110 Mustard Weed)
   BSD2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N terminus chloroplast transit peptide truncation

   Promoter

   T7

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG, I253A, H310A, H435A

   Plasmid

   #222853

   Purpose

   Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc lacking FcRn binding

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   IgG1_Fc

   Expression

   Mammalian

   Mutation

   L234A, L235A, P329G, H435A, I253A, H310A

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. hTln1-R12DD-SCLS

   Plasmid

   #191443

   Purpose

   expresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397

   Depositor

   Ben Goult

   Article

   Elefant et al JCI Insight. 2024 Dec 20;9(24):e173664.

   Insert

   Human Talin1 R12DD (TLN1 Human)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   deletion between residues K2375 and A2397

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet

   Plasmid

   #209411

   Purpose

   Expression of wild-type adult isoform of human SCN8A.

   Depositor

   Al George

   Article

   Vanoye et al JCI Insight. 2024 May 21;9(12):e177530.

   Insert

   SCN8A Variant 3, stabilized (SCN8A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Modifed human HSPA5 intron 4 inserted after bp237…

   Promoter

   CMV

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA4 3xFLAG Halo G-Linker nsp1

   Plasmid

   #175426

   Purpose

   Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   G-linker nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   G-linker in place of the RNase destabilization do…

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4 3xFLAG Halo R119A K120A nsp1

   Plasmid

   #175428

   Purpose

   Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 R119A K120A
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminal mutants R119A K120A

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA4 3xFLAG Halo E36A E37A nsp1

   Plasmid

   #175429

   Purpose

   Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 E36A E37A
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminal mutants E36A E37A

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/TO-BcRF1-ORF24-2xStrep

   Plasmid

   #138454

   Purpose

   Expresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   BcRF1-ORF24 (BcRF1, ORF24 EBV/KSHV)
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   BcRF1 amino acids 1-178 fused to ORF24 amino acid…

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA4/TO-UL87-ORF24-2xStrep

    Plasmid

    #138455

    Purpose

    Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    UL87-ORF24 (UL87, ORF24 HCMV/KSHV)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    UL87 amino acids 1-248 fused to ORF24 amino acids…

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid

    #153445

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG). Argi…

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid

    #153444

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG). Argi…

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-AAV2-hSyn-MerMAID1-Citrine

    Plasmid

    #126520

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID1
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mutation

    Promoter

    human synapsin

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4/TO-ORF66 C423A-2xStrep

    Plasmid

    #131111

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 423 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA4/TO-ORF66 C399A-2xStrep

    Plasmid

    #131112

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 399 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4/TO-ORF66 C393A-2xStrep

    Plasmid

    #131113

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 393 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4/TO-ORF66 C355A-2xStrep

    Plasmid

    #131114

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 355 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4/TO-ORF66 C344A-2xStrep

    Plasmid

    #131115

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 344 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO-ORF66 C341A-2xStrep

    Plasmid

    #131116

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C341A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 341 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO-ORF66 C301A-2xStrep

    Plasmid

    #131117

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C301A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 301 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge