Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,110 results for: NSI;

Showing: 1721 - 1740 of 12110 results

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pCDNA4TO-3XFLAG Halo R99A nsp1

Plasmid

#175422

Purpose

Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 R99A nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X-ORF24-NTD-3L_A

Plasmid

#138466

Purpose

Expresses MBP-tagged ORF24-NTD 3L_A in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

MBP

Expression

Bacterial

Mutation

Amino acids 1-201; amino acids L73-75 mutated to …

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pQLink-GST-2xCTD

Plasmid

#138470

Purpose

Expresses GST-tagged 2xCTD heptapeptide repeat in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Tags

GST

Expression

Bacterial

Mutation

2x CTD repeat

Promoter

T5

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L73A_L75A-3xFLAG

Plasmid

#138429

Purpose

Expresses full-length ORF24 L73A/L75A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L73, L75 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L74A_L75A-3xFLAG

Plasmid

#138430

Purpose

Expresses full-length ORF24 L74A/L75A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L74, L75 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 R68A-3xFLAG

Plasmid

#138431

Purpose

Expresses full-length ORF24 R68A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid R68 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 R72A-3xFLAG

Plasmid

#138432

Purpose

Expresses full-length ORF24 R72A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid R72 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L73A_L74A-3xFLAG

Plasmid

#138433

Purpose

Expresses full-length ORF24 L73A/L74A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L73, L74 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-UL87 3L_A-2xStrep

Plasmid

#138437

Purpose

Expresses full-length UL87 3L_A with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87 (UL87 HCMV (HHV-5))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids L90-L92 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1 3L_A-2xStrep

Plasmid

#138439

Purpose

Expresses full-length BcRF1 3L_A with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids L64-L66 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 3L_A-2xStrep

Plasmid

#138440

Purpose

Expresses full-length ORF24 3L_A with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids L73-L75 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 202-752-3xFLAG

Plasmid

#138424

Purpose

Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 202-752 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 202-752

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 3L_A-3xFLAG

Plasmid

#138425

Purpose

Expresses full-length ORF24 3L_A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L73-L75 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L73A-3xFLAG

Plasmid

#138426

Purpose

Expresses full-length ORF24 L73A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid L73 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L74A-3xFLAG

Plasmid

#138427

Purpose

Expresses full-length ORF24 L74A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid L74 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L75A-3xFLAG

Plasmid

#138428

Purpose

Expresses full-length ORF24 L75A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid L75 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)

Plasmid

#153439

Purpose

E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.XDocB

Tags

3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-PtenN276S

Plasmid

#140737

Purpose

Lentiviral vector to overexpress the PTEN mutation N276S as a GFP-PTEN fusion protein

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

PTEN – phosphatase and tensin homolog (PTEN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

N276S

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-2xStrep-ORF66 1-200

Plasmid

#130954

Purpose

Expresses KSHV ORF66 a.a. 1-200 with an N-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Tags

2xStrep

Expression

Mammalian

Mutation

ORF66 a.a. 1-200

Promoter

CMV

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSB153 - pL2_pSB90_2x35S::ZCT1::tMAS

Plasmid

#123195

Purpose

binary plant vector for transient expression of ZCT1 from Catharanthus roseus

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::ZCT1::tMAS

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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Showing: 1721 - 1740 of 12110 results

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