We narrowed to 4,736 results for: donor

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[#GS24 -LV] Syn1-GFP-OMP25

Plasmid

#233566

Purpose

Lentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

eGFP (eGFP Aequorea victoria, Synthetic)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pDNR-mCherry-T2A-3xPuroR-Frame 1

Plasmid

#206992

Purpose

Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDNR-mCherry-T2A-3xPuroR-Frame 2

Plasmid

#206993

Purpose

Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

Plasmid

#184397

Purpose

Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin-PTC39

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

PTC in beta-globin at amino acid position 39

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

Plasmid

#160458

Purpose

Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Inserts

PAX7 Left/5' Homology Arm (PAX7 Human)
PAX7 Right/3' Homology Arm (PAX7 Human)

Use

Insertion of a fluorescent reporter through homol…

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p15_attB(bxb)_lox

Plasmid

#183764

Purpose

Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-D-LexA::p65

Plasmid

#133562

Purpose

Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

LexA::p65

Use

CRISPR

Expression

Insect

Promoter

20X UAS

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-I-LexA::p65

Plasmid

#133563

Purpose

Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

LexA::p65

Use

CRISPR

Expression

Insect

Promoter

20X UAS

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221 MLK1

Plasmid

#60540

Purpose

Donor vector for human MLK1

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway Cloning

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
qTAG-N-Solo-mScarlet-LMNB1

Plasmid

#207771

Purpose

Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

Plasmid

#193465

Purpose

Dn29 Donor with promoter for mcherry and puro expression

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Dn29_attP

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Human 3' HP1a AID GFP PuroR

Plasmid

#127906

Purpose

HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

HP1 (CBX5 Human, Mustard Weed)

Use

Donor plasmid

Tags

GFP, AID, PuroR

Mutation

Inserting GFP AID 2A Puro into mouse 3' Hp1a

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

Plasmid

#62954

Purpose

Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGen2.1-redCALWY-1

Plasmid

#50739

Purpose

Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

Depositor

Maarten Merkx

Article

Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

Insert

gene for redCALWY-1 Zn2+ sensor

Expression

Mammalian

Available Since

Jan. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pUC19-Blast-P2A-dTAG

Plasmid

#251478

Purpose

This vector serves as a PCR template to generate donor DNA that is used for knocking-in dTAG for conditional protein degradation.

Depositor

Tomoharu Kanie

Article

Liu et al bioRxiv 2026.05.19.726384

Insert

dTAG

Use

Synthetic Biology

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19-Blast-P2A-IKZF3d

Plasmid

#251479

Purpose

This vector serves as a PCR template to generate donor DNA that is used for knocking-in IKZF3d for conditional protein degradation.

Depositor

Tomoharu Kanie

Article

Liu et al bioRxiv 2026.05.19.726384

Insert

IKZF3d

Use

Synthetic Biology

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19-Blast-P2A-HaloTag

Plasmid

#251480

Purpose

This vector serves as a PCR template to generate donor DNA that is used for knocking-in HaloTag for conditional protein degradation.

Depositor

Tomoharu Kanie

Article

Liu et al bioRxiv 2026.05.19.726384

Insert

HaloTag7

Use

Synthetic Biology

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19 KGA HA5'-mKO2-3'

Plasmid

#110406

Purpose

Homology donor for glutaminase KGA isoform knock-in, mKO2

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

CRISPR

Promoter

none

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-Puro

Plasmid

#227262

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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