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We narrowed to 4,798 results for: pcr

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Showing: 1721 - 1740 of 4798 results

  3. pSG424 ELKC(S383A)

    Plasmid
    #89334
    Purpose
    Elk1 cDNA encoding aa 307-428, aa383 mutated from ser-Ala, The PCR product was cut with BamH1 and Xba 1(365bp). pSG424 was cut with Bam H1 and Xba1. The Elk1 fragment was ligated into the BamH1
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.
    Insert
    ELK1 (ELK1 Human)
    Tags
    Gal 4
    Expression
    Mammalian
    Mutation
    encodes aa 307-428, changed serine 383 to alanine…
    Available Since
    April 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    89334-plasmid-map-sequence-id-176433

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  12. mutpRL-TK 3xUTR

    Plasmid
    #40758
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    3 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available Since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pJL258

    Plasmid
    #243697
    Purpose
    A piggyBac vector containing the endogenous NDUFB9 genomic locus, with its native 5' UTR replaced by the Actb 5' UTR, modified with silent mutations to allow for PCR-based analysis
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    NDUFB9 genomic locus, with its 5' UTR replaced by the ACTB 5' UTR (NDUFB9 Human)
    Expression
    Mammalian
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243697-plasmid-map-sequence-id-489319

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  18. pJL259

    Plasmid
    #243698
    Purpose
    A piggyBac vector containing the endogenous NDUFB9 genomic locus, with its native 3' UTR replaced by the Actb 3' UTR, modified with silent mutations to allow for PCR-based analysis
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    NDUFB9 genomic locus, with its 3' UTR replaced by the ACTB 3' UTR (NDUFB9 Human)
    Expression
    Mammalian
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243698-plasmid-map-sequence-id-489323

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  19. pJL347

    Plasmid
    #243707
    Purpose
    A piggyBac vector containing the COX5B coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs.
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    UTRs and CDS of COX5B (COX5B Human)
    Expression
    Mammalian
    Promoter
    endogenous promoter
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243707-plasmid-map-sequence-id-489300

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Showing: 1721 - 1740 of 4798 results