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We narrowed to 2,612 results for: ubiquitin

Showing: 1721 - 1740 of 2612 results

  1. His8:MBP-tev-Arg-eK-Flv

    Plasmid
    #83385
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83385-plasmid-map-sequence-id-173975

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  2. His8:MBP-tev-Phe-eK-Flv

    Plasmid
    #83384
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83384-plasmid-map-sequence-id-173972

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  3. CEAS (C-SWAT for Essential Allele Strains)

    Plasmid Kit
    #1000000223
    Purpose
    A suite of Saccharomyces cerevisiae strain construction vectors for perturbing gene expression at the transcriptional, post-transcriptional, and post-translational levels.
    Depositor
    Rob Mitra
    Available since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only

  4. p667-UBC-GFP-V5-TurboID_IDG-K

    Plasmid
    #135241
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5-TurboID
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135241-plasmid-map-sequence-id-268090

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  5. p663-UBC-TurboID-V5-dsRed_IDG-K

    Plasmid
    #135261
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135261-plasmid-map-sequence-id-268068

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  6. p663-UBC-TurboID-V5-eGFP_IDG-K

    Plasmid
    #135264
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135264-plasmid-map-sequence-id-268064

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  7. p663-UBC-miniTurbo-V5-eGFP_IDG-K

    Plasmid
    #135263
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135263-plasmid-map-sequence-id-268067

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  8. p667-UBC-GFP-V5-miniTurbo_IDG-K

    Plasmid
    #135240
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5-miniTurbo
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135240-plasmid-map-sequence-id-268088

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  9. p667-UBC-hcRed-V5-miniTurbo_IDG-K

    Plasmid
    #135237
    Purpose
    Gateway destination clone of hcRed (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    hcRed-V5-miniTurbo
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135237-plasmid-map-sequence-id-311572

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  10. p663-UBC-V5-BioID-STOP_IDG-K

    Plasmid
    #158163
    Purpose
    Gateway destination clone of BioID (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-BioID
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    158163-plasmid-map-sequence-id-328021

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  11. DTX3L

    Plasmid
    #28266
    Depositor
    Cheryl Arrowsmith
    Insert
    DTX3L (DTX3L Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains only carboxyl terminal domain (residues …
    Promoter
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    28266-plasmid-map-sequence-id-463306

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  12. p667-UBC-hcRed-V5-TurboID_IDG-K

    Plasmid
    #135238
    Purpose
    Gateway destination clone of hcRed (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    hcRed-V5-TurboID
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135238-plasmid-map-sequence-id-268556

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  13. pSR23: pET-SUMO-USP21 (1-565)

    Plasmid
    #240189
    Purpose
    Bacterial expression for USP21 (1-565)
    Depositor
    Cynthia Wolberger
    Insert
    USP21 (USP21 Human)
    Use
    Tags
    6x-His-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    240189-plasmid-map-sequence-id-476850

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  14. USP7 delta C-terminus

    Plasmid
    #234074
    Purpose
    Expresses USP7 mutant in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 1-1080 (USP7 Human)
    Use
    Tags
    6xHis cleavable with thrombin
    Expression
    Bacterial
    Mutation
    E547 mutated to stop codon
    Promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234074-plasmid-map-sequence-id-464915

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  15. p663-UBC-miniTurbo-V5-dsRed_IDG-K

    Plasmid
    #135260
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    135260-plasmid-map-sequence-id-311150

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  16. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

    Plasmid
    #131133
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-EGFP-cMycBirA*
    Use
    Lentiviral
    Tags
    NLS, HA, cMyc (N-terminal of BirA*)
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131133-plasmid-map-sequence-id-255501

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  17. His8:MBP-tev-Leu-eΔK-Flv

    Plasmid
    #83397
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83397-plasmid-map-sequence-id-175072

    Enlarge

  18. His8:MBP-tev-Arg-eΔK-Flv

    Plasmid
    #83396
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83396-plasmid-map-sequence-id-175052

    Enlarge

  19. His8:MBP-tev-Phe-eΔK-Flv

    Plasmid
    #83395
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83395-plasmid-map-sequence-id-175037

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  20. Nav1.5_1-2linker411-717_PPSYtoAASA1960-1996

    Plasmid
    #187732
    Purpose
    Express a chimera of the Nav1.5 linker between the first and second transmembrane domain with the motif surrounding PPSY; residues 1974-1977 PPSY mutated to AASA
    Depositor
    Sandra Gabelli
    Article
    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi
    Insert
    Nav1.5 1,2linker residues 411-717 and Nav1.5 residues 1960-1996 PPSY mutated to AASA (SCN5A Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    deletion of residues 1-410 and 718-1959 and 1997-…
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    187732-plasmid-map-sequence-id-372456

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Showing: 1721 - 1740 of 2612 results