We narrowed to 15,105 results for: pET

1. pETM-60-NcQDE2_517-938_H

   Plasmid

   #146459

   Purpose

   Bacterial Expression of NcQDE2_517-938

   Depositor

   Elisa Izaurralde

   Article

   Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

   Insert

   NcQDE2_517-938
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b (I-WcaINhis) K226I

   Plasmid

   #195620

   Purpose

   Expresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K226I mutation

   Depositor

   Frederick Gimble

   Insert

   I-WcaI
   

   Expression

   Bacterial

   Mutation

   A1930T, A1931T

   Promoter

   T7

   Available Since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b (I-WcaINhis) K125I

   Plasmid

   #195619

   Purpose

   Expresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K125I mutation

   Depositor

   Frederick Gimble

   Insert

   I-WcaI
   

   Expression

   Bacterial

   Mutation

   A2030T, A2031T

   Promoter

   T7

   Available Since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-15b(ETNPPL)

   Plasmid

   #192264

   Purpose

   Coding sequence of mouse ETNPPL is inserted in pET-15b vector

   Depositor

   Toshihide Yamashita

   Article

   Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

   Insert

   ethanolamine phosphate phospholyase (Etnppl Mouse)
   

   Tags

   His-tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET PI-SceI C-His

   Plasmid

   #195610

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag

   Depositor

   Frederick Gimble

   Article

   He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

   Insert

   PI-SceI
   

   Expression

   Bacterial

   Mutation

   T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…

   Promoter

   T7

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23PI-Sce ESARC

   Plasmid

   #195607

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

   Depositor

   Frederick Gimble

   Article

   Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

   Insert

   PI-SceI
   

   Expression

   Bacterial

   Mutation

   C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

   Promoter

   T7

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM-60-DmNot1_1147-1386_P

   Plasmid

   #147175

   Purpose

   Bacterial Expression of DmNot1_1147-1386

   Depositor

   Elisa Izaurralde

   Insert

   DmNot1_1147-1386 (Not1 Fly)
   

   Expression

   Bacterial

   Mutation

   1 silent mutation compared to the sequence given …

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM-60-DmNot1_1147-1717_P

   Plasmid

   #147176

   Purpose

   Bacterial Expression of DmNot1_1147-1717

   Depositor

   Elisa Izaurralde

   Insert

   DmNot1_1147-1717 (Not1 Fly)
   

   Expression

   Bacterial

   Mutation

   2 silent mutations compared to the sequence given…

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM-60-DmNot3_677-844_P

   Plasmid

   #147177

   Purpose

   Bacterial Expression of DmNot3_677-844

   Depositor

   Elisa Izaurralde

   Insert

   DmNot3_677-844 (Not3 Fly)
   

   Expression

   Bacterial

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM-41-HsNot1iso1_1085-2376_O

    Plasmid

    #147081

    Purpose

    Bacterial Expression of HsNot1iso1_1085-2376

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1iso1_1085-2376 (CNOT1 Human)
    

    Expression

    Bacterial

    Mutation

    1 silent mutation compared to the sequence of iso…

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM-41-HsNot1iso1_1597-2376_O

    Plasmid

    #147082

    Purpose

    Bacterial Expression of HsNot1iso1_1597-2376

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1iso1_1597-2376 (CNOT1 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM-41-HsNot1iso1_1085-1605_N

    Plasmid

    #147009

    Purpose

    Bacterial Expression of HsNot1iso1_1085-1605

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1iso1_1085-1605 (CNOT1 Human)
    

    Expression

    Bacterial

    Mutation

    1 silent mutations compared to the sequence of is…

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM-41-HsPAN3_291-687_N

    Plasmid

    #147013

    Purpose

    Bacterial Expression of HsPAN3_291-687

    Depositor

    Elisa Izaurralde

    Insert

    HsPAN3_291-687 (PAN3 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM-11-6His-DmPABPC1_J

    Plasmid

    #146640

    Purpose

    Bacterial Expression of DmPABPC1

    Depositor

    Elisa Izaurralde

    Insert

    DmPABPC1 (pAbp Fly)
    

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM-11-DmPABPC1_501-634_J

    Plasmid

    #146641

    Purpose

    Bacterial Expression of HsPABPC1_501-634

    Depositor

    Elisa Izaurralde

    Insert

    HsPABPC1_501-634
    

    Expression

    Bacterial

    Mutation

    2 silent mutations compared to the sequence given…

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM-41-HsPABPC1_271-542_J

    Plasmid

    #146645

    Purpose

    Bacterial Expression of HsPABPC1_271-542

    Depositor

    Elisa Izaurralde

    Insert

    HsPABPC1_271-542 (PABPC1 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET29a(+)::9xhis-Tse5

    Plasmid

    #192962

    Purpose

    Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    Tse5
    

    Tags

    9xhis + TEV tag

    Expression

    Bacterial

    Available Since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28 em-blue-shift.2

    Plasmid

    #191901

    Purpose

    Encodes a GFP design, em-blue-shift.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    em-blue-shift.2
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S S72A T108I Y145F V150I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a Cdk2ap1CAN-MutTER

    Plasmid

    #178033

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    Changed T(108)A, E(109)A, R(110)A

    Promoter

    T7LacI

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-PLS-KP7_rpoA-U

    Plasmid

    #190974

    Purpose

    Test the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP7
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET-PLS-KP1_rpoA-U

    Plasmid

    #190955

    Purpose

    Test the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP1
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET-PLS-KP4_rpoA-U

    Plasmid

    #190964

    Purpose

    Test the U-to-C editing activity of KP4 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP4
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-PLS-KP5_rpoA-U

    Plasmid

    #190965

    Purpose

    Test the U-to-C editing activity of KP5 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP5
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pETM20-9S-DYW [rpoA]

    Plasmid

    #176220

    Purpose

    Expression of TRX-9S-DYW [rpoA]

    Depositor

    Ian Small

    Article

    Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

    Insert

    TRX-9S-DYW [rpoA]
    

    Use

    Synthetic Biology

    Tags

    TRX-His

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET-GFP-9S [rpoA]

    Plasmid

    #176221

    Purpose

    Expression of GFP-9S [rpoA]

    Depositor

    Ian Small

    Article

    Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

    Insert

    GFP-9S [rpoA]
    

    Use

    Synthetic Biology

    Tags

    His-GFP

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pETM20-avi-9S-S2 [rpoA]

    Plasmid

    #176223

    Purpose

    Expression of TRX-AviTag-9S-S2 [rpoA]

    Depositor

    Ian Small

    Article

    Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

    Insert

    TRX-Avi-9S-S2 [rpoA]
    

    Use

    Synthetic Biology

    Tags

    TRX-His-AviTag

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET28-MBP-CI2F

    Plasmid

    #191254

    Purpose

    Expresses chymotrypsin inhibitor with an N-terminal MBP fusion protein

    Depositor

    Arthur Laganowsky

    Article

    Raab et al J Am Chem Soc. 2020 Oct 14;142(41):17372

    Insert

    Chymotrypsin Inhibitor 2
    

    Tags

    Maltose Binding Protein

    Expression

    Bacterial

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET24a-LaM4-2xTS

    Plasmid

    #162779

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, T7, and TS site from proCCK

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET28a-matNODALvar-HIS

    Plasmid

    #115265

    Purpose

    For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL variant mature peptide (NODAL Human)
    

    Tags

    6His

    Expression

    Bacterial

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET21a Sa-iPGM-bio

    Plasmid

    #179523

    Purpose

    Expresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coli

    Depositor

    James Inglese

    Article

    van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

    Insert

    S. aureus iPGM bio-6His
    

    Tags

    His tag and biotinylation sequence

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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