We narrowed to 275,312 results for: des

1. pcDNA pDEST40 Cas9-POLD3

   Plasmid

   #183194

   Purpose

   Expression clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   POLD3
   

   Use

   CRISPR

   Tags

   Cas9WT

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT/FLAG/HA-DEST EIF2C2

   Plasmid

   #19888

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Oct 31. ():.

   Insert

   Eukaryotic translation initiation factor 2C, 2 (AGO2 Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

   Plasmid

   #172535

   Purpose

   Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   -3.5ubb:plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F and R415H mutations obse…

   Promoter

   zebrafish ubb

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LC301: pMVP/PB/Zeo-DEST

   Plasmid

   #121877

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFRT/FLAG/HA-DEST LIN28A

   Plasmid

   #43797

   Depositor

   Thomas Tuschl

   Article

   Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

   Insert

   LIN28A (LIN28A Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFRT/FLAG/HA-DEST LIN28B

   Plasmid

   #43798

   Depositor

   Thomas Tuschl

   Article

   Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

   Insert

   LIN28B (LIN28B Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LC101: pMVP/PB/Neo-DEST

   Plasmid

   #121875

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST-CMV GFP-GABARAPL1 WT

   Plasmid

   #123117

   Purpose

   Expresses EGFP-GABARAPL1 wild-type in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST_FLAG-HUWE1_UBA/UIM-mut

   Plasmid

   #187152

   Purpose

   mammalian cell expression of FLAG-HUWE1 M1328A/F1330A/A1376G/S1382A

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   M1328A/F1330A/A1376G/S1382A

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST40-2XHA-Src-Y419F

    Plasmid

    #140316

    Purpose

    Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Y419F

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAdCMV/V5-DEST-STAT3C-3xFlag

    Plasmid

    #99264

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Expression

    Mutation

    A662C N664C

    Promoter

    CMV

    Available since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDest-623 (attR4-attR3)

    Plasmid

    #161878

    Purpose

    Multisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-DTA-DEST-R3R4(R)

    Plasmid

    #141011

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    DTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCLX-pTF-R1-DEST-R2-EBR65

    Plasmid

    #45952

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE- TRE-DEST-NBioTAP

    Plasmid

    #53568

    Purpose

    Lentiviral destination vector for N -Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

    Depositor

    Mitzi Kuroda

    Article

    Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LifeAct-EGFP-APEX2 in pCSDEST2

    Plasmid

    #170523

    Purpose

    Vector for expression of LifeAct APEX2 in mammalian cells. Parton lab clone FTE

    Depositor

    Rob Parton

    Article

    Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

    Insert

    Cavin4 (Cavin4 Mouse)
    

    Use

    Tags

    APEX2-P2A-mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GLUT9-eGFP/pcDNA-DEST47

    Plasmid

    #18730

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pFRT/FLAG/HA-DEST EIF2C3

    Plasmid

    #19889

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Oct 31. ():.

    Insert

    Eukaryotic translation initiation factor 2C, 3 (AGO3 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti X2 Neo DEST (w18-1)

    Plasmid

    #17390

    Purpose

    3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Neo

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA 3.2/V5-DEST CDC42EP1 WT

    Plasmid

    #69819

    Purpose

    Expresses CDC42EP1-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    CDC42 effector protein (CDC42EP1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 221 pCS 3MT DEST

    Plasmid

    #13070

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    6x Myc Tag
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LC001: pMVP/PB/Hygro-DEST

    Plasmid

    #121876

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDest-659 (attR4-attR3)

    Plasmid

    #161883

    Purpose

    Multisite Gateway (attR4-attR3) vector for lentivirus production

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDestTol2CG2-mnx1:mCherry-PA-Rac1

    Plasmid

    #169044

    Purpose

    Destination transgenesis vector for expressing mCherry fused with an optimized photoactivatable Rac1 protein in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    PA-Rac-1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6X His and Fused with mCherry

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    3xMnx1

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid

    #104438

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    UBQ10

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAdCMV/V5-DEST-STAT3-3xFlag

    Plasmid

    #99260

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQCXIB CMV/TO DEST (w320-1)

    Plasmid

    #17400

    Purpose

    Retroviral Gateway destination vector, Expression, CMV/TO promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Retroviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-DEST42-ARF6-V5-His6

    Plasmid

    #67415

    Purpose

    Expression of hArf6 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pFRT/FLAG/HA-DEST EIF2C1

    Plasmid

    #19887

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Oct 31. ():.

    Insert

    Eukaryotic translation initiation factor 2C, 1 (AGO1 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDEST- IP2.0(codon for C.elegans)

    Plasmid

    #109216

    Purpose

    IP2.0 for C. elegans (Codon optimization and introns).

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Use

    Tags

    His-tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDEST40-2XFL-Src-Y419F

    Plasmid

    #140317

    Purpose

    Expresses Flag-tagged human Src-Y419F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Y419F

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDest-669 (attR4-attR3)

    Plasmid

    #161887

    Purpose

    Multisite Gateway (attR4-attR3) vector for lentivirus production

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDest-658 (attR4-attR2)

    Plasmid

    #161882

    Purpose

    Multisite Gateway (attR4-attR2) vector for lentivirus production

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDEST-N2H-F2-empty vector

    Plasmid

    #125552

    Purpose

    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 alone without any attached prot…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDEST-FRT-TO-GFP-PALB2 KR

    Plasmid

    #71107

    Purpose

    mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    PALB2 (PALB2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    first 8 Lysine residues mutated to Arginine (K14-…

    Promoter

    CMV/TO

    Available since

    Dec. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pcDNA pDEST40 Cas9-HMGB1

    Plasmid

    #183195

    Purpose

    Expression clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    HMGB1
    

    Use

    CRISPR

    Tags

    Cas9WT

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFRT/FLAG/HA-DEST EIF2C4

    Plasmid

    #19890

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Oct 31. ():.

    Insert

    Eukaryotic translation initiation factor 2C, 4 (AGO4 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDEST27-GST-ATXN1 FL(84Q)

    Plasmid

    #21754

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 84 Glutamines and no…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pET-deSpCas9-VP64-6xHis

    Plasmid

    #92117

    Purpose

    Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. JL202: pMVP/mCherry-DEST

    Plasmid

    #121853

    Purpose

    pMVP destination vector, empty expression vector backbone w/ mCherry selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge