We narrowed to 7,508 results for: Pet2

1. pET20b-delta133HtrA2 S400A

   Plasmid

   #16155

   Depositor

   L. Miguel Martins

   Article

   Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   S400A

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-Hook3 aa 1-160

   Plasmid

   #74607

   Purpose

   Expresses Hook domain of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-160 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET26-CNTN149S/F366A

   Plasmid

   #100165

   Purpose

   recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

   Depositor

   Seok-Yong Lee

   Article

   Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

   Insert

   Concentrative nucleoside transporter
   

   Use

   Tags

   MBP-His

   Expression

   Bacterial

   Mutation

   N149S, F366A

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b-hTIG3-1-164

   Plasmid

   #100724

   Purpose

   Full-length human TIG3 in E.coli expression vector

   Depositor

   Byung Il Lee

   Article

   Han et al Protein Expr Purif. 2010 May;71(1):103-7

   Insert

   RARRES3 (PLAAT4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-p53 (Dog 1-77)

   Plasmid

   #62060

   Purpose

   Expression of Dog p53 (residues 1-77) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

   Insert

   p53
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-77

   Promoter

   T7

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET28b-hTIG3-1-132

   Plasmid

   #100720

   Purpose

   Expression of N-terminal region of human TIG3 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Protein Expr Purif. 2010 May;71(1):103-7

   Insert

   RARRES3 (PLAAT4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29b-EPN01(∆L1+∆L2)-His

   Plasmid

   #84511

   Purpose

   E.coli version of EPN01(∆L1+∆L2)

   Depositor

   Neil King

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   EPN01(∆L1+∆L2)-His
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28A-p53 (1-73) (P27A)

   Plasmid

   #62068

   Purpose

   Expression of human p53 (1-73) (P27A) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains TAD residues 1-73; P27A

   Promoter

   T7

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Axin GID 1-2/pET29b

   Plasmid

   #16307

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Mol Cell Biol. 1999 Oct . 19(10):7147-57

   Insert

   Axin GID 1-2 (axin1.L Frog)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino acids 320-429

   Promoter

   Available since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pET23b-proMMP-7-E215Q

    Plasmid

    #29642

    Depositor

    William Parks

    Article

    Ra et al J Biol Chem. 2009 Oct 9. 284(41):27924-3

    Insert

    proMMP-7 (MMP7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E215Q Catalytically Inactive

    Promoter

    Available since

    April 29, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pET28(a)-hFX(I154F)-HA

    Plasmid

    #14992

    Depositor

    Michael Ristow

    Insert

    human frataxin cDNA (FXN Human)
    

    Use

    Tags

    AU1, HA, and HIS

    Expression

    Bacterial

    Mutation

    contains cDNA coding sequence for amino acids 88-…

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pET28-acatenin.22-906

    Plasmid

    #186460

    Purpose

    Expresses human alpha-catenin (residues 22-906) with an N-terminal His tag and a PRescission Protease cleavage site

    Depositor

    Tina Izard

    Article

    Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a(+)-NEDD4L_W4-HECT

    Plasmid

    #187710

    Purpose

    Express his-tagged NEDD4L W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 543-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-542

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a(+)-NEDD4L_W3-W4-HECT_C874only

    Plasmid

    #187711

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 with mutations (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491; mutations C702A, C776…

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET23PI-Sce ESARC

    Plasmid

    #195607

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

    Insert

    PI-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

    Promoter

    T7

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET29a(+)::9xhis-Tse5

    Plasmid

    #192962

    Purpose

    Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    Tse5
    

    Use

    Tags

    9xhis + TEV tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28 em-blue-shift.2

    Plasmid

    #191901

    Purpose

    Encodes a GFP design, em-blue-shift.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    em-blue-shift.2
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S S72A T108I Y145F V150I

    Promoter

    T7

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a Cdk2ap1CAN-MutTER

    Plasmid

    #178033

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    Changed T(108)A, E(109)A, R(110)A

    Promoter

    T7LacI

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-MBP-CI2F

    Plasmid

    #191254

    Purpose

    Expresses chymotrypsin inhibitor with an N-terminal MBP fusion protein

    Depositor

    Arthur Laganowsky

    Article

    Raab et al J Am Chem Soc. 2020 Oct 14;142(41):17372

    Insert

    Chymotrypsin Inhibitor 2
    

    Use

    Tags

    Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET24a-LaM4-2xTS

    Plasmid

    #162779

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and TS site from proCCK

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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