We narrowed to 7,541 results for: Trac
-
Plasmid#176867PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_4
UseCRISPRTagsExpressionMutationWTPromoterAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoteract1Available SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1tetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL7007
Plasmid#136946PurposeMalachite green aptamer with stability hairpin, bacterial promoterDepositorInsertmalachite green aptamer (MGA)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
LI F-G Tet
Plasmid#104524PurposeLI resistance gene moduleDepositorInsertTetracycline resistance gene
UseCloning vectorTagsExpressionMutationPromoterAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-mCFP
Plasmid#105136PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mCFP
UseTags3nmt1-mCFPExpressionBacterial and YeastMutationA206KPromoter3nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-mYFP
Plasmid#105138PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mYFP
UseTags3nmt1-mYFPExpressionBacterial and YeastMutationA206KPromoter3nmt1Available SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TryX
Plasmid#89623Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG inductionDepositorInsertTryparedoxin
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*-(ΔLF)
Plasmid#80045PurposeExpression of Vpr binding mutant EPN-01*DepositorInsertEPN-01*(L33R)
UseTagsMycExpressionMammalianMutationVpr binding site LF45 in p6 mutatedPromoterAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01(ΔI)
Plasmid#80048PurposeExpression of EPN-01 assembly mutant L33RDepositorInsertEPN-01(L33R)
UseTagsMycExpressionMammalianMutationL33RPromoterCMVAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01(ΔL1+L2)
Plasmid#80051PurposeExpression of EPN-01 mutated in both L domainsDepositorInsertEPN-01(deltaPTAP/deltaYP)
UseTagsMycExpressionMammalianMutationPTAP10AAAA, YP37AAPromoterCMVAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*(ΔI)
Plasmid#80041PurposeExpression of EPN-01 assembly mutant L33RDepositorInsertEPN-01*(L33R)
UseTagsMycExpressionMammalianMutationchanged Leu33 in I3-01 to AlaPromoterCMVAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-Zyxin-6
Plasmid#55398PurposeLocalization: Focal Adhesions, Excitation: 433, Emission: 475DepositorAvailable SinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMDJ39 - Peft-3 Cre (PATCs)
Plasmid#191381PurposeHigh efficiency CRE recombinase expression in C. elegansDepositorInsertPeft-3 Cre tbb-2 UTR
UseTagsExpressionWormMutationPromoterPeft-3Available SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX
Plasmid#167582PurposeExpress the ATP sensor GRAB_ATP1.0 in mammalian cellsDepositorAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
psPAX2-D64V
Plasmid#63586PurposepsPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.DepositorInsertPackaging plasmid for integrase deficient lentiviral vectors
UseIntegrase deficient lentiviral packaging vectorTagsExpressionMutationPromoterAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMEH303 NLS dTomato 6x high affinity MS2 loops
Plasmid#168215PurposeMammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loopsDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPerRed-mito
Plasmid#60247PurposeMitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.DepositorInsertHyPerRed-mito
UseTagsMitochondrial targeting signalExpressionMammalianMutationPromoterCMVAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only