We narrowed to 2,291 results for: Tub;

1. pHAGE2 mCherry-Rtn4HD

   Plasmid

   #86685

   Purpose

   Lentivirus to stably express fluorescent human protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   Rtn4 homology domain (RTN4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   homology domain only

   Promoter

   CMV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2 GFP-Rtn4HD

   Plasmid

   #86686

   Purpose

   Lentivirus to stably express fluorescent human protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   Rtn4 homology domain (RTN4 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   homology domain only

   Promoter

   CMV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. actc1b-mKate2-SERCA1

   Plasmid

   #109498

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   SERCA1 (atp2a1 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   Please see Depositor Comments

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized)

   Plasmid

   #199566

   Purpose

   Stable and dox. inducible expression of Trim69 Full length, isoform A (codon optimized) after retroviral-mediated gene transfer

   Depositor

   Yuxin Song, Andrea Cimarelli

   Article

   Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

   Insert

   Trim69 Full length, isoform A (codon optimized) (TRIM69 Human)
   

   Use

   Retroviral; Allows for puromycin selection

   Tags

   Flag

   Expression

   Mutation

   codon optimized

   Promoter

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_SEPT9_i1 D1-25-msfGFP

   Plasmid

   #180333

   Purpose

   mammalian expression of human SEPT9_i1 D1-25 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 D1-25

   Promoter

   CMV

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

   Plasmid

   #107730

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

   Plasmid

   #107729

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME-GcAMP5

   Plasmid

   #126569

   Purpose

   Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   GcAMP5
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLAP-BUBR1-dKARD

   Plasmid

   #114053

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1-dKARD (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR3-vsv-GCN4-dCEN-INCENP

    Plasmid

    #108510

    Purpose

    expression of vsv-GCN4-dCEN-INCENP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    GCN4-dCEN-INCENP (INCENP Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    replace CEN box for GCN4 dimerazation domain

    Promoter

    CMV

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid

    #59357

    Purpose

    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Use

    RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acids 30-33 to confer s…

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRC/RSV-rat Agt

    Plasmid

    #122104

    Purpose

    Expression of angiotensinogen

    Depositor

    John Chan

    Article

    Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

    Insert

    angiotensinogen (Agt Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV promoter

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_SEPT2 NCmut-mApple

    Plasmid

    #180321

    Purpose

    mammalian expression of human SEPT2 NCmut fused to monomeric Apple

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    SEPT2 F20D, V27D

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

    Plasmid

    #107731

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV_SEPT9_i1-i5-msfGFP

    Plasmid

    #180332

    Purpose

    mammalian expression of human SEPT9_i1-i5 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v7 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1-i5

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP-Bin1b

    Plasmid

    #176014

    Purpose

    Mammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLAP-MIS12-KARD

    Plasmid

    #114057

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    MIS12-KARD (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-mCGN-mCGNL1-Rod1-Myc

    Plasmid

    #236526

    Purpose

    For expression of chimeric CGN containing the Rod1 domain from CGNL1 (mouse) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

    Insert

    CGN-CGNL1 (Cgn Mouse)
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Str-KDEL_SBP-EGFP-ColX

    Plasmid

    #222305

    Purpose

    Synchronize the trafficking of CollagenX from the ER.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

    Insert

    Streptavidin-KDEL and CollagenX fused to SBP-EGFP (COL10A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    signal peptide of COL10A1 removed

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Str-KDEL_SBP-EGFP-gp135

    Plasmid

    #222306

    Purpose

    Synchronize the trafficking of gp135/podocalyxin from the ER.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

    Insert

    Streptavidin-KDEL and gp135 fused to SBP-EGFP (PODXL Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    signal peptide removed

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge