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pMT-Aurora B-GFP

Plasmid

#24285

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Aurora B Kinase (aurB Fly)

Tags

GFP

Expression

Insect

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
p3E-rab7a

Plasmid

#109723

Purpose

Multisite gateway vector for 3' tagging with rab7a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab7a (rab7a Zebrafish)

Use

Zebrafish plasmids

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 C131A in pcDNA3.1

Plasmid

#118214

Purpose

Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed cysteine 131 to alanine

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab32b

Plasmid

#140885

Purpose

Expression of zebrafish GFP-tagged Rab32b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab32b (rab32b Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_A0502

Plasmid

#91012

Purpose

Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9

Use

CRISPR

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pME-nCas9n-P2A-TagBFP2-pA

Plasmid

#176026

Purpose

Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nCas9n-P2A-TagBFP2

Use

Gateway entry clone

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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XMRV vp62

Plasmid

#26189

Depositor

Joseph DeRisi

Article

Urisman et al PLoS Pathog. 2006 Mar . 2(3):e25.

Insert

XMRV VP62 (gag Human)

Expression

Mammalian

Available Since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only
pIRES RED/SP M87C448Y

Plasmid

#89324

Purpose

Expression of RFP and M87C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 deleted

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CaaX(tK)

Plasmid

#109488

Purpose

Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Kras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-CaaX(tK)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pShuttle-mCherry-EB1-C

Plasmid

#37829

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 (MAPRE1 Human)

Use

Adenoviral

Tags

mCherry

Expression

Mammalian

Mutation

aa165-268 only

Promoter

CMV

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only

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EMTB-mTurquoise2

Plasmid

#137803

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Ensconsin microtubule binding domain

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-ninein coiled-coil 2

Plasmid

#73522

Purpose

Encodes the second coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 2 (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-ninein coiled-coil 1

Plasmid

#73521

Purpose

Encodes the first coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 1 (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
pStr-KDEL_APV-SBP-DsRed2-ER-5

Plasmid

#170675

Purpose

Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Inserts

APV-SBP-DsRed2-ER-5
streptavidin-KDEL

Expression

Mammalian

Promoter

IRES and T7

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A0902

Plasmid

#91026

Purpose

Module A, Promoter: AtUbi10, Gene: TREX2-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2-P2A-AtCas9

Use

CRISPR

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-shTACC3

Plasmid

#59355

Purpose

Knocks down TACC3 in mammalian cells via shRNA and co-expresses GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

EGFP

Use

RNAi

Expression

Mammalian

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pIRES RED/SP M1C448Y

Plasmid

#89323

Purpose

Expression of RFP and M1C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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actc1b- mKate2-KDEL-pA

Plasmid

#109494

Purpose

Muscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b- mKate2-KDEL-pA

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab27a

Plasmid

#109617

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab27a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab27a (rab27a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab27a

Plasmid

#109685

Purpose

Multisite gateway vector for 3' tagging with rab27a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab27a (rab27a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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