We narrowed to 2,799 results for: fas

1. pLPCX2-FLAG-Smad4

   Plasmid

   #89834

   Purpose

   Express Smad4 in mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

   Insert

   SMAD4 (SMAD4 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. CVS-N2c-jGCaMP8m

   Plasmid

   #172388

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   GCaMP8m
   

   Use

   Neurotropic virus

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-CMV-TetO2_mVenus

   Plasmid

   #113893

   Purpose

   Mammalian (inducible) expression of cytosolic mVenus

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Insert

   monomeric Venus
   

   Use

   Lentiviral

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-FlicR1

   Plasmid

   #74143

   Purpose

   Express FlicR1 preferentially in neurons

   Depositor

   Robert Campbell

   Article

   Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

   Insert

   FlicR1
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCyterm-mScarlet-I3-NCwt_N1

   Plasmid

   #189779

   Purpose

   Construct for testing oligimerization of mScarlet-I3-NCwt in mammalian cells by OSER assay

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   Cyterm-mScarlet-I3-NCwt
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-mDLX-TVA-2A-N2cG

   Plasmid

   #172364

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neurons

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   mDLX

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21 E2F1

   Plasmid

   #90313

   Purpose

   Expresses human E2F1 in bacteria

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F1 (E2F1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGREAT26

   Plasmid

   #170914

   Purpose

   GoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pEstradiol_Inducible

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CVS-N2c-nl.mCherry-FlpO

   Plasmid

   #172378

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   nuclear-localized mCherry + FlpO
   

   Use

   Neurotropic virus

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ABI_cPPVp

    Plasmid

    #119212

    Purpose

    Expresses C-terminus of plum pox virus protease (PPVp) fused to the ABI-abscisic acid responsive protein domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cPPVp in fusion with ABI
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PYL1_nPPVp

    Plasmid

    #119211

    Purpose

    Expresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nPPVp in fusion with PYL1
    

    Tags

    AU1 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. rat Kv3.1-HA

    Plasmid

    #113055

    Purpose

    Expresses rat Kv3.1 in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

    Insert

    rat Kv3.1 (Kcnc1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. FRB_nSbMVp

    Plasmid

    #118969

    Purpose

    Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nSbMVp in fusion with FRB
    

    Tags

    Myc tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET41a iGluSnFR

    Plasmid

    #119828

    Purpose

    Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. CVS-N2c-tdTomato-FlpO

    Plasmid

    #172375

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    tdTomato + FlpO
    

    Use

    Neurotropic virus

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZA8

    Plasmid

    #158486

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1
    

    Use

    Golden gate entry vector

    Mutation

    L17E, D481V, STOP codon

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. FKBP_cSbMVp

    Plasmid

    #118968

    Purpose

    Expresses C-terminus of soybean mosaic virus protease (SbMVp) fused to the FK-506 binding protein (FKBP)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cSbMVp in fusion with FKBP
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. cycLuc_PPVs

    Plasmid

    #119208

    Purpose

    Circularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with PPVp cleavage site
    

    Use

    Luciferase

    Tags

    AU1 tag

    Promoter

    CMV

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry

    Plasmid

    #83490

    Purpose

    cre-dependent CA-AMPK under EF1a promoter

    Depositor

    Dong Kong

    Article

    Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

    Insert

    CA-AMPK-2A-mCherry
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    H150R

    Available Since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. His-ZZ-TEV-SNAPf-BICD2N(1-400)

    Plasmid

    #111857

    Purpose

    N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICD2N(1-400)
    

    Tags

    His-tag, SNAPf-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    Optimised for Sf9 expression

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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