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pGS_C_3xFLAG

Plasmid

#101653

Purpose

Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, 3xFLAG tag

Tags

3xFLAG tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAFMW-AthHEN1-CM

Plasmid

#104981

Purpose

Expression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Tags

FLAG Myc

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCML 313

Plasmid

#98586

Purpose

pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Insert

csrB

Expression

Bacterial

Promoter

pLacO

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCML 314

Plasmid

#98584

Purpose

pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Inserts

CsrA
csrB

Expression

Bacterial

Promoter

pLacO

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT1

Plasmid

#79499

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT1 (WNT1 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSI HA Nup53xl

Plasmid

#86000

Purpose

mammalian expression of HA tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

HA

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSI myc Nup53xl

Plasmid

#86001

Purpose

mammalian expression of myc tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

myc

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFP88

Plasmid

#83544

Purpose

Used to evaluate the expression output of RPL15A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL15A promoter-yEGFP

Expression

Yeast

Promoter

RPL15A

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP77

Plasmid

#83538

Purpose

Used to evaluate the expression output of RPL8B promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL8B promoter-yEGFP

Expression

Yeast

Promoter

RPL8B

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFPH0

Plasmid

#83552

Purpose

Used to evaluate the expression output of TPS1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TPS1 promoter-yEGFP

Expression

Yeast

Promoter

TPS1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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