We narrowed to 102,576 results for: Mycs
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Plasmid#200712PurposePromoter region from ATPase PMA1 as Pro5U promoter module for heterologous expression of plasma membrane localized transporters in yeastDepositorInsertPMA1 promoter (PMA1 Budding Yeast)
UseSynthetic Biology; Modular cloningMutationdomesticated for MoClo cloning (removal of intern…Available SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG
Plasmid#193317PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4FLAG N-terminal tagging
Tags4FLAGExpressionBacterialAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM4_14
Plasmid#183062PurposePlasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastPromoterHREsYGP1p and RET2Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCble
Plasmid#185861PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2S
Plasmid#185865PurposeKnocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiaeDepositorInsertALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV492
Plasmid#177700PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pYL237
Plasmid#173194PurposeCRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 RNA-binding mutant
UseCRISPRExpressionMammalianAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV491
Plasmid#177699PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF3∆165N (H348E)
Plasmid#175420PurposeEncodes His-MBP-TEV-ybbR-H348E eRF3∆165NDepositorInserteRF3 (SUP35 Budding Yeast)
TagsHis-MBP-TEV and ybbRExpressionBacterialMutation∆165N, H348EPromoterT7Available SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only