We narrowed to 15,146 results for: sgrna

1. pJZC523

   Plasmid

   #62320

   Purpose

   sgRNA for yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

   Plasmid

   #172224

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_ATP1A1_G7

   Plasmid

   #173204

   Purpose

   Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIK198

   Plasmid

   #65629

   Purpose

   "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK413

   Plasmid

   #155372

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCB092 FoMV-sgMgCh

   Plasmid

   #234815

   Purpose

   To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbMgCh (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Insert

   gRNA targeting SbMgCh
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCB036 FoMV-sgGFP

   Plasmid

   #234813

   Purpose

   To express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Insert

   gRNA targeting GFP
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCY5h

   Plasmid

   #160295

   Purpose

   Yeast CRISPR plasmid targeting the hphMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCY5n

   Plasmid

   #160296

   Purpose

   Yeast CRISPR plasmid targeting the natMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCY5k

    Plasmid

    #160294

    Purpose

    Yeast CRISPR plasmid targeting the kanMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. GMR11F02-Gal4 UAS-GFP

    Plasmid

    #230907

    Purpose

    Tissue-specific expression of GFP in wing and haltere imaginal discs.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    GMR11F02Gal4-UAS

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. UAST-FRT-GFP-FRT-uMCas12a+nls1x

    Plasmid

    #230908

    Purpose

    Double inducible expression of Cas12a+ with Gal4 and Flp.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    Cas12a+
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX458-PLK4

    Plasmid

    #227310

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of PLK4 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PLK4 (PLK4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p8270 LentiCRISPR v2 hygro sgSIGIRR-3

    Plasmid

    #193979

    Purpose

    Expression of spCas9 and sgRNA targeting SIGIRR

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDIV272

    Plasmid

    #226183

    Purpose

    Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF1p (Komagataella phaffii)

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP8835

    Plasmid

    #220902

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJMP8921

    Plasmid

    #220903

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJMP8923

    Plasmid

    #220904

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJMP8932

    Plasmid

    #220906

    Purpose

    MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hLig4 guide2 in pX458

    Plasmid

    #211536

    Purpose

    sgRNA-2 against Lig4

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA hLig4 (LIG4 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hLig4 guide1 in pX458

    Plasmid

    #211535

    Purpose

    sgRNA-1 against Lig4

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA hLig4 (LIG4 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pENTR-TC9

    Plasmid

    #202088

    Purpose

    entry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-dest

    Depositor

    David Edgell

    Article

    Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

    Insert

    TevCas9 + sgRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p8271 LentiCRISPR v2 hygro sgSIGIRR-4

    Plasmid

    #193980

    Purpose

    Expression of spCas9 and sgRNA targeting SIGIRR

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT-GFP-rG1

    Plasmid

    #188967

    Purpose

    IPTG inducible GFP with sgRNA

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    GFP
    sgRNA: agtggaaaacaatgcgaccgactagt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT-GFP-rG2

    Plasmid

    #188968

    Purpose

    IPTG inducible GFP with sgRNA

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    GFP
    sgRNA: agtccatgtaatcagcgtctactagt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PHY55-mouse U6-sgLMNA

    Plasmid

    #164045

    Purpose

    U6-driven sgRNA targeting LMNA

    Depositor

    Baohui Chen

    Article

    Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

    Insert

    LMNA targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    mouse U6

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJJF496_dpy-10_CDS_sg6

    Plasmid

    #164267

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJJF495_dpy-10_CDS_sg6

    Plasmid

    #164268

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter from W05B2.8

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJJF449_dpy-10_CDS_sg1

    Plasmid

    #163866

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJJF328_sqt-3_3′UTR_sg2

    Plasmid

    #163867

    Purpose

    sgRNA targeting sqt-3 (3′UTR) in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the 3'UTR of sqt-3
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGuide-P1-O1a (EL702)

    Plasmid

    #140040

    Purpose

    CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

    Depositor

    Johan Elf

    Article

    Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

    Insert

    barcode P1 and sgRNA lacO1 array
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUC19-hsg(NT:NT)

    Plasmid

    #138260

    Purpose

    Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. sgPYM1

    Plasmid

    #105249

    Purpose

    human PYM1 sgRNA

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    sgRNA for PYM1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

34. psPAX2-D64V-NC-COM

    Plasmid

    #131226

    Purpose

    Expressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC)

    Depositor

    Baisong Lu

    Article

    Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

    Insert

    COM
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LentiGuide Cherry

    Plasmid

    #170510

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Pavel Tolar

    Article

    Newman et al biorXiv 2021.03.02.433398

    Inserts

    S. pyogenes sgRNA cassette
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. sgOpti

    Plasmid

    #85681

    Purpose

    Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

    Depositor

    Eric Lander, David Sabatini

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pdCas9-J23109

    Plasmid

    #113151

    Purpose

    Plasmid constitutively expressing dCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A & H840A

    Promoter

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMM119

    Plasmid

    #127213

    Purpose

    BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    GFP, WUS2, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMM117

    Plasmid

    #127212

    Purpose

    BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge