We narrowed to 165,879 results for: addgene

1. 8DYS

   Plasmid

   #214439

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EIF2A:PBC022-C06:C245159 (EIF2A Human)
   

   Use

   Baculovirus

   Tags

   6xHis-TEV

   Promoter

   Polyhedrin

   Available Since

   Feb. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.1-msEGFP1.8

   Plasmid

   #208619

   Purpose

   Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.

   Depositor

   Quanfu Ma

   Insert

   msEGFP1.8
   

   Expression

   Mammalian

   Mutation

   (compared with pEGFP-C1, protein level) S30R, Y39…

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ATAXIN3:1-361_BV

   Plasmid

   #209732

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   Polyhedrin

   Available Since

   Nov. 13, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Landing_Pad_8xCTS-ECFP-pA

   Plasmid

   #200242

   Purpose

   Backbone for cloning 8xCTSs (8xCRISPR-target sites)

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN_TT Linker

   Plasmid

   #192307

   Purpose

   Gateway entry vector for cloning

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   Linker
   

   Use

   Gateway entry vector

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. KIF21A

   Plasmid

   #167843

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   KIF21A (KIF21A Human)
   

   Use

   Baculovirus expression

   Tags

   6xHis-TEV

   Promoter

   Polyhedrin

   Available Since

   April 1, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. CORO6

   Plasmid

   #167845

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CORO6 (CORO6 Human)
   

   Use

   Baculovirus expression

   Tags

   6xHis-TEV

   Promoter

   Polyhedrin

   Available Since

   April 1, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. TET-pLKO-neo NXPH4-sh3

   Plasmid

   #163746

   Purpose

   Lentivirus for inducible knockdown of NXPH4 (human)

   Depositor

   Roland Friedel

   Insert

   NXPH4 (NXPH4 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TET-pLKO-neo NXPH4-sh2

   Plasmid

   #163745

   Purpose

   Lentivirus for inducible knockdown of NXPH4 (human)

   Depositor

   Roland Friedel

   Insert

   NXPH4 (NXPH4 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TET-pLKO-neo NXPH4-sh1

    Plasmid

    #163744

    Purpose

    Lentivirus for inducible knockdown of NXPH4 (human)

    Depositor

    Roland Friedel

    Insert

    NXPH4 (NXPH4 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 6OGJ

    Plasmid

    #162258

    Purpose

    Baculovirus expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    MECP2 (MECP2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pRBPJ.1.0-gDNA

    Plasmid

    #132474

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RBPJ (RBPJ Human)
    

    Use

    CRISPR

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBbi-Pur-Lyn-AktAR2-EV-D

    Plasmid

    #125200

    Purpose

    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Insert

    Lyn-AktAR2-EV-D
    

    Use

    Transposon

    Tags

    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

    Expression

    Mammalian

    Mutation

    FoxO1 Thr-24 changed to Val rendering this inacti…

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. NFATc4-CRISPR-gRNA#exon5-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124290

    Purpose

    sgRNA against huiman NFATc4

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    NFATc4 nuclear factor of activated T cells (NFATC4 Human)
    

    Use

    CRISPR

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

15. Thy-1-CRISPR-gRNA#1-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124283

    Purpose

    sgRNA against human Thy-1

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

16. Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124285

    Purpose

    sgRNA against human Thy-1

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

17. Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124284

    Purpose

    sgRNA against human Thy-1

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

18. NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124289

    Purpose

    sgRNA against human NFATc4

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    NFATc4 nuclear factor of activated T cells (NFATC4 Human)
    

    Use

    CRISPR

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

19. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid

    #118159

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid

    #118160

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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