We narrowed to 10,644 results for: cat.1

1. pQhsCDtRNA+

   Plasmid

   #86014

   Purpose

   Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene

   Depositor

   Hong Li

   Article

   Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

   Inserts

   U14-tRNA chimera (SNORD14A Human)
   15.5K (SNU13 Human)
   fibrillarin (FBL Human)
   NOP56 (NOP56 Human)
   NOP58 (NOP58 Human)
   

   Use

   Co-expression; purification

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   amino acids 1-401, amino acids 1-411, and amino a…

   Promoter

   LPP and Ptac

   Available since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-4T Syt1 C2AB(140) WT

   Plasmid

   #134695

   Purpose

   Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purification

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Synaptotagmin 1 (Syt1 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETmsGFPSyt1(80 JuxtaK-142)

   Plasmid

   #209013

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Tags

   6xHis-msfGFP

   Expression

   Bacterial

   Mutation

   aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETSUMOSyt1C2AB(80 JuxtaK-421)

   Plasmid

   #209008

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-BRIC

   Plasmid

   #172338

   Purpose

   Bioluminescent Red Indicator for Calcium

   Depositor

   Huiwang Ai

   Article

   Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

   Insert

   BRIC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-BRIC

   Plasmid

   #172343

   Purpose

   Bioluminescent Red Indicator for Calcium (bacterial expression)

   Depositor

   Huiwang Ai

   Article

   Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

   Insert

   BRIC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac1-pGC-A

   Plasmid

   #186626

   Purpose

   pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

   Depositor

   Debra Hansen

   Article

   Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

   Insert

   NPR1 (NPR1 Human)
   

   Use

   Tags

   hemagglutinin (HA) signal peptide + His10-tag + T…

   Expression

   Insect

   Mutation

   deleted amino acids 1-32

   Promoter

   polyhedring

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. L40C-CRISPR.EFS.mNeon

   Plasmid

   #69146

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_LolT_C41S

   Plasmid

   #211789

   Purpose

   Expression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41S

   Depositor

   Yang Hai

   Article

   Gao et al Chembiochem. 2023 Oct 1:e202300561. doi:

   Insert

   lolt mutant
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pColE1_sgRNA_pos7_AmpR

    Plasmid

    #119150

    Purpose

    Encodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 7 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pColE1_sgRNA_pos5_AmpR

    Plasmid

    #119148

    Purpose

    Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 5 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-BG505.SOSIP-gp160-QES.i05.c06-754*

    Plasmid

    #123275

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT2/shCol1a1/Scarlet_Seq1.3

    Plasmid

    #206137

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1 (Col1a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT2/shCol1a1/GFP4_Seq1.3

    Plasmid

    #206136

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1 (Col1a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

    Plasmid

    #209016

    Purpose

    Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETSyt1C2AB(80 JuxtaK-421)msGFP

    Plasmid

    #209011

    Purpose

    Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis and msfGFP

    Expression

    Bacterial

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T Syt1 C2AB(140) M173C

    Plasmid

    #134696

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    M173C, C277A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-4T Syt1 C2AB(140) F234C

    Plasmid

    #134697

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F234C, C277A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T Syt1 C2AB(140) V304C

    Plasmid

    #134698

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    V304C, C277A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-4T Syt1 C2AB(140) I367C

    Plasmid

    #134699

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    I367C, C277A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge