We narrowed to 2,347 results for: puc19

1. pJBL7051

   Plasmid

   #167221

   Purpose

   cell-free cadmium sensing reporter

   Depositor

   Julius Lucks

   Insert

   pCad-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJBL7053

   Plasmid

   #167222

   Purpose

   cell-free lead sensing reporter

   Depositor

   Julius Lucks

   Insert

   pPbr-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJBL7054

   Plasmid

   #167223

   Purpose

   cell-free copper sensing reporter

   Depositor

   Julius Lucks

   Insert

   pCue-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJBL7064

   Plasmid

   #167233

   Purpose

   cell-free cascaded cadmium sensor

   Depositor

   Julius Lucks

   Insert

   pCad-AKSIRV
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NK691

   Plasmid

   #120850

   Purpose

   Reporter gene expression in S. rosetta

   Depositor

   Nicole King

   Insert

   Jumblelw1-mWasabi
   

   Use

   Choanoflagellate expression

   Tags

   Expression

   Mutation

   Jumblelw1 allele: T to C at nt 1109 causes L305P …

   Promoter

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pILFPQ13BE5

   Plasmid

   #223836

   Purpose

   Expressing a cytosolic cAMP sensor (mScarletI3-ApacH-StayGold2) in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Insert

   pTEF1>mScarletI3>ApacH>StayGold2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGGC_RT_XVE

   Plasmid

   #136970

   Purpose

   Goldengate/Greengate module for generation of beta-estradiole inducible genes.

   Depositor

   Klaus Theres

   Insert

   XVE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   BsaI site domestication 834 T > A,

   Promoter

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAC32 [TU#839]

   Plasmid

   #16079

   Depositor

   Martin Chalfie

   Insert

   mec-2 intron 9
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   June 23, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGGC_RT_Sa_sgRNA

   Plasmid

   #136968

   Purpose

   Goldengate/Greengate module for genome editing, using the Sa guideRNA.

   Depositor

   Klaus Theres

   Insert

   Sa guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pwAC-trAO217erg12

    Plasmid

    #227347

    Purpose

    Overexpression of the truncated AO217 and erg12 genes in Aspergillus oryzae

    Depositor

    Jun-ichi Maruyama

    Insert

    Expression cassettes of the truncated AO217 and erg12 genes
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJBL7065

    Plasmid

    #167234

    Purpose

    cell-free cascaded nitrate sensor

    Depositor

    Julius Lucks

    Insert

    pNar-AKSIRV
    

    Use

    Synthetic Biology

    Tags

    6X His

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJBL7066

    Plasmid

    #167235

    Purpose

    cell-free cascaded mercury sensor

    Depositor

    Julius Lucks

    Insert

    pMer-AKSIRV
    

    Use

    Synthetic Biology

    Tags

    6X His

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJBL7048

    Plasmid

    #167218

    Purpose

    cell-free nitrate sensing reporter

    Depositor

    Julius Lucks

    Insert

    pNar-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ydfJ115 promoter

    Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZ3CH10-donor

    Plasmid

    #176368

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    Z3CH10
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGGA_RT_Sa_Cas9

    Plasmid

    #136962

    Purpose

    Goldengate/Greengate module for genome editing, using the SaCAS9.

    Depositor

    Klaus Theres

    Insert

    SaCAS9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGGD_RT_biChromium

    Plasmid

    #136974

    Purpose

    Goldengate/Greengate C-tag module, containing a dual fluorescent marker based on the P2A peptide

    Depositor

    Klaus Theres

    Insert

    P2A sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGGI_RT_Sa_sgRNA

    Plasmid

    #136982

    Purpose

    Goldengate/Greengate module for genome editing, using the Sa guideRNA.

    Depositor

    Klaus Theres

    Insert

    Sa guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGGD_RT_Sa_sgRNA

    Plasmid

    #136975

    Purpose

    Goldengate/Greengate module for genome editing, using the Sa guideRNA.

    Depositor

    Klaus Theres

    Insert

    Sa guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGGD_RT_li_Tq2CFP_2NLS

    Plasmid

    #136972

    Purpose

    Goldengate/Greengate C-tag module, containing Tq2CFP fused to 2 SV40 NLS and a flexible linker

    Depositor

    Klaus Theres

    Insert

    Tq2CFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGGE_RT_Sa_sgRNA

    Plasmid

    #136978

    Purpose

    Goldengate/Greengate module for genome editing, using the Sa guideRNA.

    Depositor

    Klaus Theres

    Insert

    Sa guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PB-NEO-GFP-RFP

    Plasmid

    #157952

    Purpose

    seamless gene editing and component of Piggybac transposase system

    Depositor

    Yan-zhen Bi

    Type

    Empty backbone

    Use

    Mouse Targeting; Gene targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSB-Bleo STAT3

    Plasmid

    #139184

    Purpose

    Empty backbone encoding constitutive bleomycin resistance with an inducible STAT3 promoter to allow for an insert in a Sleeping Beauty backbone.

    Depositor

    Neal Devaraj

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    STAT3

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHS201-ophi

    Plasmid

    #227349

    Purpose

    Heterologous production of ophiobolin C in Aspergillus oryzae

    Depositor

    Jun-ichi Maruyama

    Insert

    The hoomlogous regions of the HS201 locus and expression cassettes of the AcOS, oblDBm, and oblBBm genes
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pwAB-erg8erg19idi1erg20

    Plasmid

    #227345

    Purpose

    Overexpression of the erg8, erg19, idi1, and erg20 genes in Aspergillus oryzae

    Depositor

    Jun-ichi Maruyama

    Insert

    Expression cassettes of the erg8, erg19, idi1, and erg20 genes
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pdadhDN-tefAO217

    Plasmid

    #227341

    Purpose

    Replacement of the adh gene with the expression cassette of the AO217 gene

    Depositor

    Jun-ichi Maruyama

    Insert

    Flanking regions of adh (AO090009000634) and expression cassette of AO217 (AO090120000217)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ppG-ple12

    Plasmid

    #227339

    Purpose

    Heterologous production of pleuromutilin in Aspergillus oryzae

    Depositor

    Jun-ichi Maruyama

    Insert

    The upstream/downstream regions of the pyrG gene and expression cassettes of the ple1 and ple2 genes
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pwA-ple34

    Plasmid

    #227337

    Purpose

    Heterologous production of pleuromutilin in Aspergillus oryzae

    Depositor

    Jun-ichi Maruyama

    Insert

    The upstream/inside regions of the wA gene and expression cassettes of the ple3 and ple4 genes
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

    Plasmid

    #155306

    Purpose

    Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Rfx Cas13d CRISPR RNA BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTOX-donor

    Plasmid

    #132516

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TOX (TOX Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    none

    Promoter

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTBX18-donor

    Plasmid

    #176370

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    TBX18 (TBX18 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pZNF468-donor

    Plasmid

    #176350

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZNF468 (ZNF468 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUC-dirTopo-P2A-aGFP

    Plasmid

    #61974

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Promoter

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUC-dirTopo-AU1-aGFP

    Plasmid

    #61973

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Promoter

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pIAeBlue

    Plasmid

    #185891

    Purpose

    In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAW8-aid-Turg1:kanMX6

    Plasmid

    #169354

    Purpose

    C-terminal aid degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOTX2-donor

    Plasmid

    #176358

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    OTX2 (OTX2 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pIEforRed

    Plasmid

    #185892

    Purpose

    In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSTAT5B-donor

    Plasmid

    #176339

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    STAT5B (STAT5B Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMDW133

    Plasmid

    #227248

    Purpose

    Lambda DNA for torsional templates

    Depositor

    Michelle Wang

    Insert

    Lambda DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pXylP-HygR

    Plasmid

    #168131

    Purpose

    Expression of HygR from the xylose-inducible xylP promoter

    Depositor

    Michael Bromley

    Insert

    XylP-HygR
    

    Use

    Aspergillus expression

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    xylP

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pOSR1-donor

    Plasmid

    #176363

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    OSR1 (OSR1 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pZNF627-donor

    Plasmid

    #176365

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZNF627 (ZNF627 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pZNF79-donor

    Plasmid

    #176371

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZNF79 (ZNF79 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSNAPC5-donor

    Plasmid

    #176341

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    SNAPC5 (SNAPC5 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pZNF449-donor

    Plasmid

    #132515

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF449 (ZNF449 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    none

    Promoter

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLinker-2xTy-HXGPRT-LoxP

    Plasmid

    #86664

    Purpose

    CRISPR tagging with 2xTy in Toxoplamsa gondii

    Depositor

    David Sibley

    Insert

    Linker-2xTy-HXGPRT 3'UTR
    

    Use

    Expression in toxoplasma gondii

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pNPAS1-donor

    Plasmid

    #176346

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    NPAS1 (NPAS1 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pSOX15-donor

    Plasmid

    #176356

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    SOX15 (SOX15 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAW8-aid-eDSR-Turg1:kanMX6

    Plasmid

    #169362

    Purpose

    C-terminal aid-eDSR degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pUC-ODN2

    Plasmid

    #208643

    Purpose

    Empty vector for long single strand DNA (up to 1.5k) preparation. Further remove BsaI and BsmBI site in pUC-ODN (Addgene #208642).

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Long single strand dna production

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge