We narrowed to 1,166 results for: Ncl

1. ACS_14

   Plasmid

   #111649

   Purpose

   Funclib design ACS_14

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   ACS_14
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ACS_16

   Plasmid

   #111650

   Purpose

   Funclib design ACS_16

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   ACS_16
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ACS_27

   Plasmid

   #111652

   Purpose

   Funclib design ACS_27

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   ACS_27
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PTE_5

   Plasmid

   #111635

   Purpose

   Funclib design PTE_5

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   PTE_5
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTac

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PTE_13

   Plasmid

   #111636

   Purpose

   Funclib design PTE_13

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   PTE_13
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTac

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PTE_24

   Plasmid

   #111637

   Purpose

   Funclib design PTE_24

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   PTE_24
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTac

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRK5-HA-Ubiquitin-WT

   Plasmid

   #17608

   Purpose

   Mammalian expression of HA tagged ubiquitin

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JD633

   Plasmid

   #160393

   Purpose

   CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Inserts

   ZmUbi::wheat GRF4-GIF1 chimera
   ZmUbi:TaCas9 + TaU6:gRNA
   

   Use

   Binary vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDECKO_GFP

   Plasmid

   #72619

   Purpose

   Expresses two gRNAs targeting GFP

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15TV-L-hlPYK WT

    Plasmid

    #187975

    Purpose

    Expresses the human liver pyruvate kinase

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

    Insert

    hlPYK WT
    

    Use

    Tags

    Hexahistag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJMP1356

    Plasmid

    #119276

    Purpose

    for cloning new sgRNAs

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a hMDH2

    Plasmid

    #204230

    Purpose

    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    hMDH2 (MDH2 Human)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Does not include transit peptide and includes a M…

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJMP1183

    Plasmid

    #119254

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDECKO_UCA1

    Plasmid

    #72628

    Purpose

    Expresses two gRNAs targeting the UCA1 promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward UCA1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJMP1185

    Plasmid

    #119255

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJMP1237

    Plasmid

    #119262

    Purpose

    for cloning new sgRNAs

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJMP1358

    Plasmid

    #119277

    Purpose

    for cloning new sgRNAs

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDECKO_Malat1_A

    Plasmid

    #72620

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry_EHMT1_full length

    Plasmid

    #228810

    Purpose

    Expression of mouse EHMT1

    Depositor

    Shravanti Rampalli

    Article

    Biligiri et al PLOS Biology 22(11): e3002871.

    Insert

    EHMT1 (Ehmt1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    See Depositor Comments

    Promoter

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJMP1354

    Plasmid

    #119275

    Purpose

    for cloning new sgRNAs

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge