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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 579 results for: SPY

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Showing: 161 - 180 of 579 results

  4. pCG-D-mSpyCatcher-His6-E

    Plasmid
    #87376
    Purpose
    An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF ass
    Depositor
    Tom Ellis
    Article
    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021
    Insert
    mSpyCatcher
    Use
    Synthetic Biology
    Tags
    His6 tag
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87376-plasmid-map-sequence-id-183395

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  6. pCG-D-SpyTag-His6-E

    Plasmid
    #87375
    Purpose
    An ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assembly
    Depositor
    Tom Ellis
    Article
    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021
    Insert
    SpyTag
    Use
    Synthetic Biology
    Tags
    His6 tag
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87375-plasmid-map-sequence-id-183404

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  7. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid
    #107421
    Purpose
    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    SpyCatcher
    Tags
    His tag, StrepII, and superfolder GFP
    Expression
    Bacterial
    Mutation
    mutation of glutamate 77 to glutamine
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107421-plasmid-map-sequence-id-207622

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  8. pIBA2-SpyTag-MBP

    Plasmid
    #107422
    Purpose
    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    Maltose-binding protein (MalE)
    Tags
    OmpA signal peptide, SpyTag, and StrepII
    Expression
    Bacterial
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107422-plasmid-map-sequence-id-212955

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  10. mCherry-PhoCl AND A-SpyTag

    Plasmid
    #246950
    Purpose
    Expresses multifunctional construct for AND-gated release of mCherry from materials in response to 405 nm light AND sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag, SsrA
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246950-plasmid-map-sequence-id-489551

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Showing: 161 - 180 of 579 results