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Showing: 161 - 180 of 260 results

  1. pET15bLCFPT7

    Plasmid
    #53545
    Purpose
    Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant
    Depositor
    Lingchong You
    Article
    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10
    Insert
    T7 RNAP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    53545-plasmid-map-sequence-id-83442

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  4. pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7

    Plasmid
    #231353
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231353-plasmid-map-sequence-id-459833

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  5. pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7

    Plasmid
    #231357
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231357-plasmid-map-sequence-id-459837

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  6. pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7

    Plasmid
    #231358
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231358-plasmid-map-sequence-id-459844

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  7. pAAV.T7-SP6-BC(p11-14)-SCP1-CI-mRuby2-W3SL-T7

    Plasmid
    #231359
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from SCP1 promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231359-plasmid-map-sequence-id-459843

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  8. pRAV23

    Plasmid
    #67715
    Purpose
    Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification
    Depositor
    Jeffrey Kieft, Robert Batey
    Article
    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pRAV24

    Plasmid
    #67716
    Purpose
    Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification
    Depositor
    Jeffrey Kieft, Robert Batey
    Article
    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Sept. 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pAJ1

    Plasmid
    #138379
    Purpose
    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ
    Depositor
    Seth Darst
    Article
    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11
    Inserts
    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    Use
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…
    Promoter
    T7
    Available since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138379-plasmid-map-sequence-id-275999

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  12. pAIP4_3V46

    Plasmid
    #234165
    Purpose
    Heterologous protein expression of cell division control protein 73 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3V46 (CDC73 Budding Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234165-plasmid-map-sequence-id-464131

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  13. pRAV12

    Plasmid
    #67713
    Purpose
    Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification
    Depositor
    Jeffrey Kieft, Robert Batey
    Article
    Kieft et al RNA. 2004 Jun;10(6):988-95.
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67713-plasmid-map-sequence-id-126588

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  15. pTmaM4

    Plasmid
    #67714
    Purpose
    Expression vector hexahistidine tagged variant of the Thermotoga maritima M-domain protein
    Depositor
    Jeffrey Kieft, Robert Batey
    Article
    Kieft et al RNA. 2004 Jun;10(6):988-95.
    Insert
    Thermotoga marĂ­tima Ffh-M domain
    Use
    Tags
    hexahistidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67714-plasmid-map-sequence-id-126589

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  18. pAAV.T7-SP6-BC(p11-14)-mDLX-minBG-CI-mRuby2-W3SL-T7

    Plasmid
    #231352
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter with mDLX enhancer.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231352-plasmid-map-sequence-id-459729

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Showing: 161 - 180 of 260 results