We narrowed to 266 results for: T7 rna polymerase

1. pIA1363

   Plasmid

   #166861

   Purpose

   Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp8 (ORF1ab Synthetic)
   

   Tags

   His-GB1

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIA1362

   Plasmid

   #166860

   Purpose

   Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp7 (ORF1ab Synthetic)
   

   Tags

   His-GB1

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. DH10B-ALT

   Bacterial Strain

   #61151

   Purpose

   DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Bacterial Resistance

   None

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

4. C98m

   Plasmid

   #121002

   Purpose

   MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Gp2 toxin
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pARiBo1

   Plasmid

   #98892

   Purpose

   Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

   Depositor

   Pascale Legault

   Article

   Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

   Insert

   ARiBo1
   

   Use

   In vitro transcription vector

   Promoter

   T7 promoter

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15bLCFPT7

   Plasmid

   #53545

   Purpose

   Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant

   Depositor

   Lingchong You

   Article

   Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

   Insert

   T7 RNAP
   

   Expression

   Bacterial

   Available Since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIA1385

   Plasmid

   #166862

   Purpose

   Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PVS10

   Plasmid

   #104398

   Purpose

   polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7

   Plasmid

   #231353

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7

    Plasmid

    #231357

    Purpose

    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7-SP6-BC(p11-14)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7

    Plasmid

    #231358

    Purpose

    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7-SP6-BC(p11-14)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.T7-SP6-BC(p11-14)-SCP1-CI-mRuby2-W3SL-T7

    Plasmid

    #231359

    Purpose

    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from SCP1 promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7-SP6-BC(p11-14)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid

    #239293

    Purpose

    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

    Depositor

    Anita Hopper

    Article

    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

    Inserts

    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    

    Expression

    Yeast

    Mutation

    Contains M2-SE-G72P mutations, described in Addge…

    Promoter

    TDH3 and tetracycline-responsive element (TRE) an…

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRAV23

    Plasmid

    #67715

    Purpose

    Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification

    Depositor

    Robert Batey, Jeffrey Kieft

    Article

    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

    Insert

    Transcription cassette for 3'-tagged RNAs
    

    Expression

    Bacterial

    Promoter

    T7 RNA polymerase

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pRAV24

    Plasmid

    #67716

    Purpose

    Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification

    Depositor

    Robert Batey, Jeffrey Kieft

    Article

    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

    Insert

    Transcription cassette for 3'-tagged RNAs
    

    Expression

    Bacterial

    Promoter

    T7 RNA polymerase

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pAJ1

    Plasmid

    #138379

    Purpose

    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ

    Depositor

    Seth Darst

    Article

    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11

    Inserts

    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…

    Promoter

    T7

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNG1256

    Plasmid

    #149710

    Purpose

    Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

    Depositor

    Peter Lewis

    Article

    Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

    Insert

    rpoZ (rpoZ )
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAIP4_3V46

    Plasmid

    #234165

    Purpose

    Heterologous protein expression of cell division control protein 73 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3V46 (CDC73 Budding Yeast)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pRAV12

    Plasmid

    #67713

    Purpose

    Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification

    Depositor

    Robert Batey, Jeffrey Kieft

    Article

    Kieft et al RNA. 2004 Jun;10(6):988-95.

    Insert

    Transcription cassette for 3'-tagged RNAs
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7 RNA polymerase

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHMM

    Plasmid

    #67717

    Purpose

    Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

    Depositor

    Robert Batey, Jeffrey Kieft

    Article

    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

    Insert

    Hexahistidine/Maltose binding protein/MS2 coat protein fusion
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7 RNA polymerase

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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