We narrowed to 14,176 results for: TIM

1. Flag‐hTim1

   Plasmid

   #49207

   Purpose

   expresses Flag-tagged human Tim1

   Depositor

   Lawrence Kane

   Article

   de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

   Insert

   Tim1 (HAVCR1 Human)
   

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   Tim1 lacks its own signal sequence

   Promoter

   EF1a

   Available Since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

2. STIMATE-mCherry

   Plasmid

   #70676

   Purpose

   Expresses STIMATE or TMEM110 in mammalian cells

   Depositor

   Yubin Zhou

   Article

   Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

   Insert

   STIMATE (TMEM110 Human)
   

   Tags

   mcherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pGEX6P1-optimizedNedd4.2d

   Plasmid

   #165103

   Purpose

   bacterial expression of a GST fusion of Human Nedd4.2d

   Depositor

   Arthur Haas

   Article

   Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

   Insert

   Nedd4.2d (NEDD4L Human)
   

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MultiMate 5 colors

   Plasmid

   #206266

   Purpose

   Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
   

   Use

   Recombinant baculovirus production, cre acceptor…

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiMutB3GNT5-blast

   Plasmid

   #208380

   Purpose

   lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   nt 922 c->g (PAM site inactivation)

   Promoter

   EF-1 alpha core promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiMutB3GALT5-blast

   Plasmid

   #208381

   Purpose

   lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   nucleotide change in PAM targeting sequence nt 51…

   Promoter

   EF-1 alpha core promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiMutB3GALT5catdead-blast

   Plasmid

   #220869

   Purpose

   lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   encodes D156A and D243A mutations; also, nucleoti…

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLNCX2-STIM1

   Plasmid

   #89817

   Purpose

   Express HUMAN STIM1

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Retroviral

   Tags

   NO TAG

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFPN1‐mTim1

   Plasmid

   #49215

   Purpose

   expresses EGFP tagged mouse Tim1

   Depositor

   Lawrence Kane

   Insert

   Tim1 (Havcr1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pETDuet-1 TIM8,13

    Plasmid

    #170282

    Purpose

    Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

    Insert

    yeast Tim8 and Tim13 (TIM8 Budding Yeast)
    

    Tags

    TEV-cleavable N-terminal His tag on Tim13 (on bac…

    Expression

    Bacterial

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag‐mTim1 (Bl/6)

    Plasmid

    #49205

    Purpose

    expresses Flag tagged mouse Tim1

    Depositor

    Lawrence Kane

    Article

    de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

    Insert

    Tim1 (BL/6 allele) (Havcr1 Mouse)
    

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    Tim1 lacks its own signal sequence

    Promoter

    EF1a

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pOpen-NotIM

    Plasmid

    #165518

    Purpose

    Site-specific DNA methyltransferase; protects DNA from cleavage by NotI

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    M.NotI
    

    Use

    Synthetic Biology

    Available Since

    July 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. STIMATE(1-240)

    Plasmid

    #71280

    Purpose

    Expresses STIMATE or TMEM110 (1-240) in mammalian cells

    Depositor

    Yubin Zhou

    Article

    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

    Insert

    STIMATE (1-240) (TMEM110 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

14. Stim1-CaRQ

    Plasmid

    #34560

    Depositor

    Kevin Truong

    Article

    Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

    Insert

    Stim1-CaRQ (STIM1 Human)
    

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Mutation

    Q63L in RhoA

    Promoter

    CMV, T7

    Available Since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

15. Flag‐Tim2

    Plasmid

    #49213

    Purpose

    expresses Flag tagged Tim2

    Depositor

    Lawrence Kane

    Article

    Knickelbein et al J Immunol. 2006 Oct 15;177(8):4966-70.

    Insert

    Tim2 (Timd2 Mouse)
    

    Tags

    CD8 signal sequence and FLAG

    Expression

    Mammalian

    Mutation

    no Tim2 signal sequence; R80L and S196D compared …

    Promoter

    EF1a

    Available Since

    Dec. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pLAS2w.Phyg-TIMD4

    Plasmid

    #232670

    Purpose

    expresses TIMD4 in mammalian cells

    Depositor

    Chen-Yang Shen

    Insert

    TIMD4 (TIMD4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. STIM1-2Strep

    Plasmid

    #210361

    Purpose

    Expresses STIM1 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a(+)-HisTim12_CtoA

    Plasmid

    #170281

    Purpose

    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

    Insert

    yeast Tim12 (TIM12 Budding Yeast)
    

    Tags

    TEV-cleavable N-terminal His tag (on backbone)

    Expression

    Bacterial

    Mutation

    mutated two non-conserved Cys to Ala

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P1-optimizedNedd4.2F823D

    Plasmid

    #165105

    Purpose

    bacterial expression of a GST fusion of Human Nedd4.2d F823D

    Depositor

    Arthur Haas

    Article

    Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

    Insert

    Nedd4.2d F823D (NEDD4L Human)
    

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    codon optimized for E. coli expression; F823D; …

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFS_0367_pET30-RT-CRISPR_Woodsholea_maritima_ARRAY_1

    Plasmid

    #116979

    Purpose

    Expresses WmRT-Cas1-Cas2 under pT7lac promoter, encodes WmCRISPR array 1, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Woodsholea maritima RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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