We narrowed to 932 results for: multisite gateway

1. pME-FastFUCCI (JDW 1488)

   Plasmid

   #242556

   Purpose

   Gateway middle entry clone containing FastFUCCI reporter; For visualizing cell cycle state

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
   

   Use

   Gateway subcloning

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-WPRE-SV40-pA (JDW 922)

   Plasmid

   #224545

   Purpose

   A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   WPRE-SV40-pA
   

   Use

   Gateway cloning

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME Actin-Vhh sfGFP (JDW 1215)

   Plasmid

   #224495

   Purpose

   Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-mScarlet3-Giantin (JDW 1356)

   Plasmid

   #224502

   Purpose

   Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet3-Giantin
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME EFS-rtTA/rtTA3 (JDW 1230)

   Plasmid

   #224525

   Purpose

   A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EFS-rtTA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME Actin Vhh Halo Tag (JDW 1223)

   Plasmid

   #224498

   Purpose

   Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-Halo-Tag
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-Lifeact-EGFP-3xHA (JDW 1322)

   Plasmid

   #224494

   Purpose

   Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Liefact-EGFP-3xHA
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-DRE-nls (JDW 31)

   Plasmid

   #242561

   Purpose

   Gateway middle entry clone containing Dre recombinase

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   codon optimized DRE-nls
   

   Use

   Gateway subcloning

   Available Since

   Sept. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-EGFP-miR-MCS (JDW 1231)

   Plasmid

   #242562

   Purpose

   Gateway middle entry clone containing an EGFP fused to a microRNA multiple cloning site.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EGFP with artifial intron and MCS
   

   Use

   Gateway subcloning

   Available Since

   Sept. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-Lifeact-mRuby2-3xMyc (JDW 1246)

    Plasmid

    #242554

    Purpose

    Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mRuby2
    

    Use

    Gateway subcloning

    Available Since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-zFUCCI (JDW 1464)

    Plasmid

    #242558

    Purpose

    Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)
    

    Use

    Gateway subcloning

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-H2B-mStayGold-WPRE (JDW 1384)

    Plasmid

    #242550

    Purpose

    Gateway middle entry clone containing H2B-mStayGold with WPRE at the 3' end; Nuclear green fluorescent reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mStayGold (monomeric StayGold)
    

    Use

    Gateway subcloning

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-H2B-mBaoJin-WPRE (JDW 1383)

    Plasmid

    #242549

    Purpose

    Gateway middle entry clone containing H2B-mBaoJin with WPRE at the 3' end; Nuclear green fluorescent reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mBaoJin
    

    Use

    Gateway subcloning

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-H2B-mScarlet-3H (JDW 1513)

    Plasmid

    #242551

    Purpose

    Gateway middle entry clone containing H2B-mScarlet-3H; Nuclear red fluorescent reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet3-H
    

    Use

    Gateway subcloning

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-mCerulean3-TUBB1A (JDW 1508)

    Plasmid

    #242555

    Purpose

    Gateway middle entry clone containing mCerulean3-TUBB1A; Cyan fluorescent reporter for visualizing tubulin in cells

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mCerulean3-TUBB1A
    

    Use

    Gateway subcloning

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME nls EGFP WPRE bGH pA (JDW 488)

    Plasmid

    #224540

    Purpose

    A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-EGFP-WPRE-bGH-pA
    

    Use

    Gateway cloning

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME nls BFP FLAG WPRE bGH pA (JDW 484)

    Plasmid

    #224539

    Purpose

    A Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleus

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-mTagBFP2-FLAG_WPRE-bGH-pA
    

    Use

    Gateway cloning

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3E-EF1a-pA (JDW 1222)

    Plasmid

    #224526

    Purpose

    A Gateway compatible 3' entry clone containing rat EF-1a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EF-1a polyA
    

    Use

    Gateway cloning

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-WPRE-bGH-pA (JDW 1221)

    Plasmid

    #224527

    Purpose

    A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    WPRE-bGH
    

    Use

    Gateway cloning

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5E-dr_Unc (JDW 1365)

    Plasmid

    #224482

    Purpose

    Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Unc-503 promoter
    

    Use

    Gateway cloning

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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