We narrowed to 25,227 results for: nes

1. pCAGGS-6015nes

   Plasmid

   #108657

   Purpose

   A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-S6K-EV
   

   Tags

   XFP

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. piGECI-NES-N1

   Plasmid

   #160422

   Purpose

   Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI-NES
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETSUMO2_Rhodoplanes-bGSDM

   Plasmid

   #212129

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Rhodoplanes bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mPlum-Farnesyl-5

   Plasmid

   #55968

   Purpose

   Localization: Plasma Membrane, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. BirA*-HA-NES

   Plasmid

   #214473

   Purpose

   Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   BirA(R118G)
   

   Use

   Lentiviral; Gateway

   Tags

   HA tag and NES (Nuclear Exclusion Sequence)

   Expression

   Mammalian

   Mutation

   R118G

   Promoter

   EF-1a

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mTFP1-Farnesyl-5

   Plasmid

   #55481

   Purpose

   Localization: Plasma Membrane, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   Farnesyl (HRAS Human)
   

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. nes714tk/lacZ

   Plasmid

   #47614

   Purpose

   used to create transgenic mice expressing LacZ reporter with Nestin enhancer

   Depositor

   Urban Lendahl

   Article

   Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.

   Insert

   nestin 2nd intron fragment (NES Human)
   

   Use

   Enhancer reporter

   Tags

   HSV tk promoter and lacZ

   Mutation

   714 bp from 3' end of 2nd intron

   Promoter

   HSV tk promoter

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

8. mWasabi-Farnesyl-5

   Plasmid

   #56503

   Purpose

   Localization: Plasma Membrane, Excitation: 493, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Tags

   mWasabi

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pAS_MmaPylT_EF1_NES-MmaPylRS AF

   Plasmid

   #174902

   Purpose

   expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG and NES

   Expression

   Mammalian

   Mutation

   Y306A, Y384F

   Promoter

   EF1

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. L_NES 67kDa

    Plasmid

    #201357

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Adeno_NES-EGFP-6PrA
    

    Expression

    Mammalian

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. H_NES 67kDa

    Plasmid

    #201350

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    HIV_NES-EGFP-6PrA
    

    Expression

    Mammalian

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. M_NES 67kDa

    Plasmid

    #201356

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    MAPK_NES-EGFP-6PrA
    

    Expression

    Mammalian

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mIFP12-Farnesyl-5

    Plasmid

    #56251

    Purpose

    Localization: Plasma Membrane, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    mIFP12

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. mPapaya1-Farnesyl-5

    Plasmid

    #56650

    Purpose

    Localization: Membrane, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    mPapaya1

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEos3.2-Farnesyl-5

    Plasmid

    #57456

    Purpose

    Localization: Membrane, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCSIIpuro-4493NES

    Plasmid

    #175811

    Purpose

    The lentiviral vector for Booster-PKA, a Red-Shifted genetically encoded FRET biosensor for monitoring PKA activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-PKA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. miniSOG-Farnesyl-5

    Plasmid

    #62782

    Purpose

    Localization: Membrane, Excitation:448 / 473, Emission:500 / 528

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. Dronpa-Farnesyl-5

    Plasmid

    #57272

    Purpose

    Localization: Membrane, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    Dronpa

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. mTurquoise-Farnesyl-5

    Plasmid

    #55551

    Purpose

    Localization: CAAX - Membrane, Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    mTurquoise

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pPBbsr2-SCAT3NES

    Plasmid

    #197350

    Purpose

    Encoding SCAT3NES.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    SCAT3NES
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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