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pH6HTC_hsHUR-Halo

Plasmid

#175303

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HUR (ELAVL1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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1.6 kB VEGF-luc

Plasmid

#29667

Depositor

Patricia D'Amore

Article

Shima et al J Biol Chem. 1996 Feb 16. 271(7):3877-83

Insert

1.6 kB VEGF

Use

Luciferase

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
NODALvar-CAG-AAVS1

Plasmid

#115262

Purpose

For stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cells

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1-internal6xHis

Plasmid

#60390

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4)

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pH6HTC_IGF2BP2b-Halo

Plasmid

#175318

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP2 (IGF2BP2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Isoform B

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pME-fli1b

Plasmid

#49019

Purpose

full length coding sequence for zebrafish fli1b gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

friend leukemia integration 1b (fli1rs Zebrafish)

Use

Gateway middle entry

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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800 pME-Bozozok

Plasmid

#49008

Purpose

full length coding sequence for zebrafish bozozok gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

dharma (dharma )

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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LSM102 CMV-TO-CX3CR1-10aa-PCP (FLP-IN)

Plasmid

#233404

Purpose

CX3CR1-V2-PCP with a 10 amino acid linker and V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CX3CR1-PCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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APL0041 Ac5-FRB-MCP

Plasmid

#233412

Purpose

AC5 promoter driven FRB-MCP for drosophila cell expression

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FRB-MCP

Expression

Mammalian

Mutation

WT

Promoter

Ac5

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ076 (PB) CMV-mCCL20 (PuroR-BFP)

Plasmid

#233433

Purpose

PiggyBac donor plasmid, membrane-bound CCL20

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20-pre-mGRASP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ033 CMV-TO-CCL20-pre-mGRASP

Plasmid

#233417

Purpose

plasmid expressing membrane-bound CCL20 by fusing with pre-mGRASP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20-pre-mGRASP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0031 SFFV-AVPR2-ADAR2dd (FLP-IN)

Plasmid

#233437

Purpose

SFFV driven AVPR2-ADAR2dd (as in XZ030)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR2-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0030 SFFV-bArrestin2-MCP (FLP-IN)

Plasmid

#233436

Purpose

SFFV driven ARRB2-tdMCP with a short linker

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ARRB2-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0043 SFFV-CCR6-ADAR2dd (FLP-IN)

Plasmid

#233438

Purpose

SFFV driven CCR6-ADAR2dd (as in XZ032)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ073 (IVT) CMV-TO-T7-LIDARsensor-hybridUTR3

Plasmid

#233420

Purpose

IVT template for LIDAR reporter mRNA; mCherry as marker, EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-LIDARsensor-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ089 (IVT) CMV-TO-T7-ARRB2-MCP-hybridUTR3

Plasmid

#233422

Purpose

IVT template for ARRB2-tdMCP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-ARRB2-MCP-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM052 CMV-TO-CCR6-PCP (FLP-IN)

Plasmid

#233406

Purpose

CCR6-V2-PCP with a 4 amino acid linker and V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-PCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0040 Ac5-FKBP-ADARdd

Plasmid

#233411

Purpose

AC5 promoter driven FRBP-ADAR2dd for drosophila cell expression

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

Ac5

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ038 CMV-TO-IGF1R-ADAR2(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233391

Purpose

IGF1R-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IGF1R-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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YH038 PGK-TO-FRB-tm-MCP

Plasmid

#233377

Purpose

PGK promoter driven FRB fused with MCP, with a CD28 transmembrane domain in between

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FRB-CD28TM-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

PGK

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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