We narrowed to 29,262 results for: NTS;

1. pET21c(+)-mtLeuRS

   Plasmid

   #31079

   Depositor

   Linda Spremulli

   Article

   Bullard et al Biochim Biophys Acta. 2000 Feb 29. 1490(

   Insert

   Mature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Nt …

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

   Plasmid

   #205268

   Purpose

   Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1 omegaRNA
   

   Expression

   Mammalian

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

   Plasmid

   #205268

   Purpose

   Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1 omegaRNA
   

   Expression

   Mammalian

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNL4-3 envFS eGFP Δvif/vpr

   Plasmid

   #101344

   Purpose

   Deletion from NL4-3 nt 5582-6199 encompassing Vif and Vpr coding sequence

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Mutation

   delta vif/Vpr

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNL4-3 envFS eGFP Δvif/vpr

   Plasmid

   #101344

   Purpose

   Deletion from NL4-3 nt 5582-6199 encompassing Vif and Vpr coding sequence

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Mutation

   delta vif/Vpr

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-mCherry-CyrCR1

   Plasmid

   #128768

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-mCherry-CyrCR1

   Plasmid

   #128768

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. hum sgk promoter F4R4

   Plasmid

   #83426

   Purpose

   promoter reporter construct to test 5' flanking sequences

   Depositor

   Christie Thomas

   Article

   Itani et al Am J Physiol Endocrinol Metab. 2002 Nov;

   Insert

   human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Promoter

   sgk

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. hum sgk promoter F4R4

   Plasmid

   #83426

   Purpose

   promoter reporter construct to test 5' flanking sequences

   Depositor

   Christie Thomas

   Article

   Itani et al Am J Physiol Endocrinol Metab. 2002 Nov;

   Insert

   human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Promoter

   sgk

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. ptagRFP-MS2coatProtein

    Plasmid

    #103831

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    MS2 coat protein (MCP) (cp )
    

    Tags

    tagRFP

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. ptagRFP-MS2coatProtein

    Plasmid

    #103831

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    MS2 coat protein (MCP) (cp )
    

    Tags

    tagRFP

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

    Plasmid

    #205278

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

    Plasmid

    #205278

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Fireworks RFP[PTC-] (AVA2515)

    Plasmid

    #85443

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Fireworks RFP[PTC-] (AVA2515)

    Plasmid

    #85443

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-dSa-VPR mini.-2X snRP1

    Plasmid

    #99688

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-dSa-VPR mini.-2X snRP1

    Plasmid

    #99688

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmKate2-MS2coatProtein

    Plasmid

    #103833

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mKate2-MS2 coat protein (cp )
    

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmKate2-MS2coatProtein

    Plasmid

    #103833

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mKate2-MS2 coat protein (cp )
    

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiMutB3GALT5-blast

    Plasmid

    #208381

    Purpose

    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nucleotide change in PAM targeting sequence nt 51…

    Promoter

    EF-1 alpha core promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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