Addgene

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pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

Plasmid

#31481

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (Sce target) EF BFP

Use

Lentiviral

Tags

Expression

Mutation

mCherry contains M9S and M16L to reduce backgroun…

Promoter

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pSL3402 (pTnsABf_Pse)

Plasmid

#200901

Purpose

Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseTnsA-TnsB

Use

Tags

Internal NLS between TnsA-TnsB fusion

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK-HA-TRAF5

Plasmid

#58315

Purpose

HA-tagged mouse TRAF5

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 5 (Traf5 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG LAP2

Plasmid

#15738

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti-TetO-PM

Plasmid

#197387

Purpose

To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

PDX1-T2A-MAFA

Use

Lentiviral; Teto

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe puro Snail

Plasmid

#23347

Depositor

Bob Weinberg

Article

Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

Insert

Snail (SNAI1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-KLF5

Plasmid

#40918

Depositor

Jin-Tang Dong

Article

Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

Insert

Kruppel-like factor 5 (KLF5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pN1-CMV-sDarken

Plasmid

#184799

Purpose

Serotonin Sensor sDarken under the control of a CMV Promoter.

Depositor

Olivia Masseck

Article

Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

Insert

sDarken

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-ATBF1

Plasmid

#40927

Depositor

Jin-Tang Dong

Article

Dong et al J Biol Chem. 2010 Oct 22;285(43):32801-9

Insert

ATBF1 (ZFHX3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

CMV

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pC3.1_CAG_oROS-Gr

Plasmid

#216113

Purpose

Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.01.31.578117

Insert

CAG_oROS-Gr

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-GpNLuc

Plasmid

#70185

Purpose

Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects..

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro LAP2

Plasmid

#15740

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 0819

Plasmid

#118704

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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sIL6R PLAK1

Plasmid

#161519

Purpose

Expresses soluble IL6 receptor in mammalian cells.

Depositor

Yoav Shaul

Article

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

soluble IL-6 receptor

Use

Lentiviral

Tags

3X Flag

Expression

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET21 MBPx-SpyCatcher

Plasmid

#72327

Purpose

Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET21 MBPx-SpyCatcher

Use

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

MBP contains A312V, I317V mutations and 172, 173,…

Promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 3809

Plasmid

#118705

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSL0341 (pTarget)

Plasmid

#200906

Purpose

Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

pTarget

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-SpyCas9

Plasmid

#113033

Purpose

SpyCas9 expression in mammalian cells (CMV promoter driven)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SpyCas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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mCer-4aa-VAMP2

Plasmid

#53233

Purpose

Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

VAMP2 (Vamp2 Rat)

Use

Tags

4aa-linker and mCerulean

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only