We narrowed to 29,083 results for: PLE;

1. pLenti CMVtight eGFP Neo (w784-1)

   Plasmid

   #26586

   Purpose

   2nd generation lentiviral vector

   Depositor

   Eric Campeau

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral; Tet-on advanced vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti CMV/TO p16 Hygro (w184-1)

   Plasmid

   #22263

   Depositor

   Eric Campeau

   Insert

   cyclin-dependent kinase inhibitor 2A (CDKN2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX307-SARS-CoV-2-3CL-C145A

   Plasmid

   #160279

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C145A

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-Puro-PNPT1 S484A

   Plasmid

   #223316

   Purpose

   Lentiviral vector expressing human PNPT1 S484A variant

   Depositor

   Judy Lieberman

   Article

   Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

   Insert

   Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed serine 484 to alanine

   Promoter

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-tdTomato-miRE_shRen-Puro

   Plasmid

   #135687

   Purpose

   Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

   Depositor

   Florian Karreth

   Insert

   shRenilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-FHH-NRASQ61K-IRES-Puro

   Plasmid

   #120570

   Purpose

   Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMV Puro DHFR.dn-cHSF1

   Plasmid

   #134729

   Purpose

   Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   DHFR.dn-cHSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Tags

   DHFR

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-202, 379−529

   Promoter

   CMV

   Available since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

   Plasmid

   #20946

   Purpose

   3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   halorhodopsin
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-TRE3G-BE3RA-PGK-Puro

   Plasmid

   #110846

   Purpose

   Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   TRE3G

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EF1alpha-IRES-EGFP-HA-hNPHP1

    Plasmid

    #122874

    Purpose

    Expresses HA-tagged human NPHP1 in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Insert

    human NPHP1 (NPHP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX-FHH-NRAS-IRES-Puro

    Plasmid

    #120569

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti GW V5 Eco/Dam mse EMD

    Plasmid

    #182675

    Purpose

    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.

    Depositor

    Bas van Steensel

    Article

    Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    E. Coli Dam and V5

    Expression

    Mammalian

    Mutation

    Promoter

    HSP

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225698

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K27M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Mutation

    K27M

    Promoter

    pEF1A

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-Cre-MS2_UbC-GFP (Cre_GFP)

    Plasmid

    #219536

    Purpose

    a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLenti CMV/TO V5-LUC Puro (w549-1)

    Plasmid

    #19785

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

    Plasmid

    #71216

    Purpose

    dCas9 fused to human DNMT3A catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3A catalytic domain
    

    Use

    Lentiviral

    Tags

    dSpCas9-DNMT3A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CaMKIIa-eNpHR 3.0-EYFP

    Plasmid

    #26970

    Purpose

    3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Insert

    eNpHR 3.0
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Trafficking Signal (TS), ER Export Signal (FCYENE)

    Promoter

    CaMKIIa

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiPGK Puro DEST JNKKTR AA Clover

    Plasmid

    #90238

    Purpose

    Lentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter (AA mutant)
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CMV-CAG-GFP-EnvA

    Plasmid

    #102987

    Purpose

    Lentivirus expressing eGFP and (EnvA-B19 Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    Enhanced Green Fluorescent Protein (eGFP)
    EnvA-B19 Rabies Glycoprotein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti GW V5 Eco/Dam mse LaminA

    Plasmid

    #182674

    Purpose

    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.

    Depositor

    Bas van Steensel

    Article

    Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:

    Insert

    Lamin A (Lmna Mouse)
    

    Use

    Lentiviral

    Tags

    E. Coli Dam and V5

    Expression

    Mammalian

    Mutation

    Promoter

    HSP

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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