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pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

Plasmid

#84240

Purpose

Expresses GA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pN3-MGA

Plasmid

#107715

Purpose

Expression plasmid for 3xFLAG-tagged MGA

Depositor

Guntram Suske

Article

Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

Insert

MGA (MGA Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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CBEd SpG UGI Puro

Plasmid

#235045

Purpose

Cytosine base editor d with SpG nicking Cas9 makes C > T edits

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

CBEd, nCas9

Use

CRISPR and Lentiviral

Tags

P2A-PuroR

Promoter

EF1a core

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-nsp14

Plasmid

#168874

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-nsp14 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-nsp9

Plasmid

#168872

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-nsp9 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP/golgin-160

Plasmid

#180630

Purpose

Expression of WT golgin-160

Depositor

Carolyn Machamer

Article

Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

Insert

golgin-160 (GOLGA3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

Plasmid

#84260

Purpose

Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgSV40
PYL1-KRAB

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Promoter

CMV and mouse U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR4BN (Lenti)

Plasmid

#222692

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

PGK without BsmBI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-RspCas13d_RspCasWYL1

Plasmid

#108304

Purpose

Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Inserts

RspCas13d
RspWYL1

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-HsSAS6

Plasmid

#136790

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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