We narrowed to 11,201 results for: NSI

1. pDESTFLAGHApuro3-Ago Chi-a

   Plasmid

   #14075

   Depositor

   Thomas Tuschl

   Article

   Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

   Insert

   Ago Chi-a
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Ago Chimera-a

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pDZ529 pTRP MET25p PP7-PS-2x-yeGFP

   Plasmid

   #72233

   Purpose

   Tryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   PP7-PS-2x-yeGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p6671 MSCV-IP N-HAonly 38E6

   Plasmid

   #44144

   Depositor

   Peter Howley

   Article

   White et al J Virol. 2012 Dec;86(24):13174-86. doi:

   Insert

   HPV38E6
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   April 30, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pDESTFLAGHApuro3-Ago Chi-b

   Plasmid

   #14085

   Depositor

   Thomas Tuschl

   Article

   Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

   Insert

   Ago Chi-b
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Ago Chimera-b

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pDESTFLAGHApuro3-Ago Chi-c

   Plasmid

   #14086

   Depositor

   Thomas Tuschl

   Article

   Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

   Insert

   Ago Chi-c
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Ago Chimera-c

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pLL3.7 Alk.1 shRNA

   Plasmid

   #59296

   Purpose

   shRNA to mouse Alk

   Depositor

   Ulrike Heberlein

   Article

   Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

   Insert

   Alk.1 shRNA
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCSP1-HA-xEcto

   Plasmid

   #20900

   Depositor

   Stefano Piccolo

   Article

   Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

   Insert

   Ectodermin (trim33 Frog)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   contains full unsequenced 3' UTR

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pET3a-AqAdk_V145C

   Plasmid

   #18091

   Depositor

   Dorothee Kern

   Article

   Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

   Insert

   adenylate kinase (kad A. aeolicus)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Val 145 changed to Cys

   Promoter

   Available since

   May 29, 2008

   Availability

   Academic Institutions and Nonprofits only

9. Cenp-B INCENP Δcen mCherry

   Plasmid

   #45227

   Depositor

   Michael Lampson, Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   vsv Cenp-B INCENP Δcen mCherry
   

   Use

   Tags

   Expression

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pDESTFLAGHApuro3-Ago Chi-d

    Plasmid

    #14087

    Depositor

    Thomas Tuschl

    Article

    Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

    Insert

    Ago Chi-d
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Ago Chimera-d

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pL-rpb1-D260N

    Plasmid

    #10985

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-D260N (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 778 G to A in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pL-rpb1-D261N

    Plasmid

    #10986

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-D261N (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 781 G to A in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pL-rpb1-R320C

    Plasmid

    #10987

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-R320C (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 958 C to T in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pL-rpb1-E1230K

    Plasmid

    #10990

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-E1230K (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 3688 G to A in the RPB1 ORF resulting in…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pSLB027

    Plasmid

    #234636

    Purpose

    pET-28a(+) based plasmid for expression of H16_B1102 from Cupriavidus necator H16, with N-terminal hexahistidine tag

    Depositor

    Gregg Beckham

    Article

    Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

    Insert

    H16_B1102 (H16_RS24270 )
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLB028

    Plasmid

    #234637

    Purpose

    pET-28a(+) based plasmid for expression of H16_B1148 from Cupriavidus necator H16, with N-terminal hexahistidine tag

    Depositor

    Gregg Beckham

    Article

    Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

    Insert

    H16_B1148 (H16_RS24495 )
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLB029

    Plasmid

    #234638

    Purpose

    pET-28a(+) based plasmid for expression of H16_B1264 from Cupriavidus necator H16, with N-terminal hexahistidine tag

    Depositor

    Gregg Beckham

    Article

    Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

    Insert

    H16_B1264 (H16_RS25065 )
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSLB030

    Plasmid

    #234639

    Purpose

    pET-28a(+) based plasmid for expression of H16_B1335 from Cupriavidus necator H16, with N-terminal hexahistidine tag

    Depositor

    Gregg Beckham

    Article

    Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

    Insert

    H16_B1335 (H16_RS25400 )
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLB024

    Plasmid

    #234633

    Purpose

    pET-28a(+) based plasmid for expression of H16_A0285 from Cupriavidus necator H16, with N-terminal hexahistidine tag

    Depositor

    Gregg Beckham

    Article

    Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

    Insert

    H16_A0285 (H16_RS01400 Cupriavidus necator H16)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLB026

    Plasmid

    #234635

    Purpose

    pET-28a(+) based plasmid for expression of H16_A3514 from Cupriavidus necator H16, with N-terminal hexahistidine tag

    Depositor

    Gregg Beckham

    Article

    Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

    Insert

    H16_A3514 (H16_RS17530 )
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPB-CAG-dCas9-VPR-pgk-hph

    Plasmid

    #233066

    Purpose

    Piggybac CRISPRa construct

    Depositor

    Markus Landthaler

    Article

    Vučićević et al bioRxiv 2024.09.12.612198

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCHW00010

    Plasmid

    #222435

    Purpose

    Level 0, promoter module (GGAG-AATG) containing CBS4 and tomato miniDFR promoters.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    CBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYPQAT08

    Plasmid

    #223380

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYPQAT09

    Plasmid

    #223381

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYPQAT10

    Plasmid

    #223382

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYPQAT01

    Plasmid

    #223373

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYPQAT02

    Plasmid

    #223374

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYPQAT04

    Plasmid

    #223376

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYPQAT07

    Plasmid

    #223379

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. POSV801-SP44-SnaAinBCDEOT1T2

    Plasmid

    #225056

    Purpose

    Heterologous expression in Streptomyces with inactive SnaA

    Depositor

    Wilfred van der Donk

    Article

    Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

    Insert

    inactivated SnaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S556A/S1616A

    Promoter

    SP44

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. POSV801-SP44-SnaABCDEinOT1T2

    Plasmid

    #225057

    Purpose

    Heterologous expression in Streptomyces with inactive SnaE

    Depositor

    Wilfred van der Donk

    Article

    Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

    Insert

    inactivated SnaE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deltaA46-V801

    Promoter

    SP44

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt

    Plasmid

    #233240

    Purpose

    Parallel coiled-coil with (GGS)3 linker to prone condensation

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    Vh-clover-GCN4-(GGS)3-Vt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt

    Plasmid

    #233241

    Purpose

    Parallel coiled-coil with (GGS)12 linker to prevent condensation

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    Vh-clover-GCN4-(GGS)12-Vt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pP4-C

    Plasmid

    #225097

    Purpose

    gfpUV reporter vector with a high performance adenine-dependent riboswitch

    Depositor

    Robert Batey

    Article

    Drogalis et al PLoS One. 2020 Dec 1;15(12):e0243155. do

    Insert

    synthetic riboswitch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-AzaQ

    Plasmid

    #224429

    Purpose

    Express AzaQ in E. coli

    Depositor

    Emily Balskus

    Article

    Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

    Insert

    azaQ
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBbA2C_Ptet::luxI

    Plasmid

    #189574

    Purpose

    Lux synthase (3OC6-HSL)

    Depositor

    Daniel Ducat

    Article

    Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

    Insert

    Acyl-homoserine-lactone synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBbA2C_Ptet::traI

    Plasmid

    #189575

    Purpose

    Tra synthase (3OC8-HSL)

    Depositor

    Daniel Ducat

    Article

    Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

    Insert

    Acyl-homoserine-lactone synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBbA2C_Ptet::lasI

    Plasmid

    #189576

    Purpose

    Las synthase (3OC12-HSL)

    Depositor

    Daniel Ducat

    Article

    Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

    Insert

    Acyl-homoserine-lactone synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCHW91015

    Plasmid

    #222455

    Purpose

    Level 1, Position 2 containing Mas::CUP2-VP16::NOS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    Mas::CUP2-VP16::NOS (CUP2 )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDest17_A1-LCD swap V1

    Plasmid

    #226538

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD V1

    Depositor

    Tanja Mittag

    Article

    Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

    Insert

    A1-LCD swap V1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge