We narrowed to 2,870 results for: GFP reporter gene

1. pRluc-LC3wt

   Plasmid

   #105002

   Purpose

   Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pN1-Lck-GCaMP3

   Plasmid

   #26974

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

   Insert

   Lck-GCaMP3
   

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Plasmid contains N-terminal 26 amino acid membran…

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV 4xSTAR-mTagBFP2-blast

   Plasmid

   #125239

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

   Insert

   stem cell Ascl2 reporter, 4 repeats
   

   Use

   Lentiviral

   Tags

   mTagBFP2 (codon optimized)

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-CMV-Nluc

   Plasmid

   #73038

   Purpose

   Express Nluc reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGF1-4eCOL2A1

   Plasmid

   #97210

   Purpose

   Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter

   Depositor

   Ryan Porter

   Article

   De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

   Insert

   4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   COL2A1 regulatory elements

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-iCas9-neo

   Plasmid

   #85400

   Purpose

   Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Insert

   Flag-iCas9-P2A-GFP
   

   Use

   Lentiviral

   Tags

   Flag-iCas9-P2A-GFP

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CMV-puro_FlnaABD-TS-16flag

   Plasmid

   #234868

   Purpose

   Expresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.

   Depositor

   Michael Murrell

   Article

   Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

   Insert

   FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16
   

   Use

   Lentiviral

   Tags

   GFP, RFP, FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV6-AC 4-μHOM

   Plasmid

   #113619

   Purpose

   Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   4-μHOM variant of EGFP
   

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. LEPG

   Plasmid

   #111160

   Purpose

   miR-E (miR-30 variant)-based RNAi with GFP reporter

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Mutation

   WT

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. TC1405

    Plasmid

    #164877

    Purpose

    BFP-preSTOP-GFP reporter

    Depositor

    Tian Chi

    Article

    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

    Insert

    BFP-preSTOP-GFP
    

    Expression

    Mammalian

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. VanGlow-GL

    Plasmid

    #83342

    Purpose

    for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

    Depositor

    Cheng-Yu Lee

    Article

    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    GFP::Luciferase(nls)

    Expression

    Insect

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD/HisB-Medium-FT

    Plasmid

    #31917

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    araB

    Available Since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOIMars1CyN

    Plasmid

    #75221

    Purpose

    Mammalian cell surface expression of fluorogen activating protein Mars1Cy

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    ss-HA-Mars1Cy-CD80(237-306)
    

    Tags

    HA epitope

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Lyso-ExRai-AMPKAR

    Plasmid

    #192449

    Purpose

    ExRai-AMPKAR targeted to the lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    Lyso-ExRai-AMPKAR
    

    Tags

    Full-length LAMP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-mTagBFP2-FRB-Rab5

    Plasmid

    #214272

    Purpose

    Mammalian expression of mTagBFP2-FRB-Rab5 (early endosome marker)

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    mTagBFP2-FRB-Rab5A (RAB5A Canis lupus familiaris (Dog))
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. BII-C3H2B

    Plasmid

    #133387

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRluc-LC3G120A

    Plasmid

    #105003

    Purpose

    Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Mutation

    Glycine 120 mutated to alanine, prevents lipidati…

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EF1-Nluc

    Plasmid

    #73024

    Purpose

    Express Nluc reporter under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPSint

    Plasmid

    #149421

    Purpose

    Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene.

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTHSSd_8

    Plasmid

    #59948

    Purpose

    PT7 GFP reporter

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    sfGFP
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PT7

    Available Since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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