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Plasmid#20957Purpose3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, PuroDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only
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Myog'-2A-mCherryNLS-PGK-Puro
Plasmid#69548PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…ExpressionMutationSilent V205V mutation in myogenin gene to prevent…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-5xA
Plasmid#91992PurposeExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)PromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet pLKO.1-shCSE1L v1 puro
Plasmid#192345PurposeLentiviral expression vector for an inducible shCSE1L v1DepositorInsertshCSE1L v1 (CSE1L Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet pLKO.1-shCSE1L v2 puro
Plasmid#192346PurposeLentiviral expression vector for an inducible shCSE1L v2)DepositorInsertshCSE1L v2 (CSE1L Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroR
Plasmid#190076Purposeexpress human ACE2 with selectable markers iRFP670 and puromycin resistance geneDepositorInsertACE2 (ACE2 Human)
UseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shFAK #1
Plasmid#198757Purposeconditional knockdown of FAKDepositorInsertshFAK #1 (PTK2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shFAK #2
Plasmid#198758Purposeconditional knockdown of FAKDepositorInsertshFAK #2 (PTK2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSHiFi-P2A-Puro
Plasmid#136902PurposeLentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)DepositorInsertFNLSHiFi
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationR691APromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
1039 pBabe puroL PTEN A4
Plasmid#10941DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutationA4: S380A, T382A, T383A, S385APromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL L188V-pBabe-puro
Plasmid#19241DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Leucin 188 to ValinPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1RA-P2A-Puro
Plasmid#110853PurposeLentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGExpressionMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-PuroR-HA
Plasmid#163352PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection markerDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-M2rtTA-T2A-Puro
Plasmid#169175PurposepiggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174DepositorInsertM2rtTA-T2A-Puro
UsePiggybac transposonTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
OCT4 GFP puro Donor 3
Plasmid#22211DepositorInsertSA-OCT-GFP-2APuro-PA (POU5F1 Human)
UseTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-puroR-p2a-HA-halo
Plasmid#161979Purposehomologous donor template for endogenous taggingDepositorInsertpuroR-p2a-HA-halo-GSlinker
UseTagsExpressionMutationPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro shPOLE1_1
Plasmid#160810PurposeExpress Dox repressible shPOLE1DepositorInsertshPOLE1_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
plko-puromycin-shTRX1-259
Plasmid#110921PurposeTo knockdown Thioredoxin-1DepositorInsertTRX1 (TXN Human)
UseLentiviralTagsExpressionMutationPromoterunknownAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-Flag-TRAF5
Plasmid#102679Purposeretroviral expression plasmid for TRAF5DepositorInsertTRAF5 (Traf5 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Myog'-2A-mCherryNLS-PGK-Purodtk
Plasmid#69547PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…ExpressionMutationSilent V205V mutation in myogenin gene to prevent…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only