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pCMV7.1_3xFLAG-WWC2

Plasmid

#172995

Purpose

constitutive expression of FLAG-tagged WWC2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC2 (WWC2 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc

Plasmid

#208851

Purpose

Expresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGCP123-GFP_g1

Plasmid

#153518

Purpose

sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-GeNL

Plasmid

#208841

Purpose

Expresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

GeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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GDAP2-c005

Plasmid

#173110

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GDAP2 (GDAP2 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

M1-N231

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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STIMATE-mCherry

Plasmid

#70676

Purpose

Expresses STIMATE or TMEM110 in mammalian cells

Depositor

Yubin Zhou

Article

Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

Insert

STIMATE (TMEM110 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pCMV7.1_3xFLAG-WWC3

Plasmid

#172996

Purpose

constitutive expression of FLAG-tagged WWC3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC3 (WWC3 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Article

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Tags

luciferase

Expression

Bacterial

Available Since

April 24, 2006

Availability

Academic Institutions and Nonprofits only
Tmem214 pmRFP-N2 (1348)

Plasmid

#62047

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
Tmem214 pEGFP-N2 (1072)

Plasmid

#62026

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
5UTR_MpUBE2

Plasmid

#136110

Purpose

5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

5UTR_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pANTO5

Plasmid

#131106

Purpose

To generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integrase

Depositor

Riccardo Manganelli

Article

Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

Insert

tetR gene by pFRA61 (Boldrin et al 2010)

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT-TO-GFP-PALB2 KR

Plasmid

#71107

Purpose

mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

GFP

Expression

Mammalian

Mutation

first 8 Lysine residues mutated to Arginine (K14-…

Promoter

CMV/TO

Available Since

Dec. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFUGW-RapACR-EYFP

Plasmid

#111075

Purpose

Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RapACR

Use

Lentiviral

Tags

EYFP

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL3Sc

Plasmid

#109365

Purpose

Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-MCPIP1(delta ZF)

Plasmid

#90110

Purpose

Mammalian expression of Flag-MCPIP1(delta ZF)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

Flag

Expression

Mammalian

Mutation

K317G, S318G

Promoter

CMV

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET19b Adrm1

Plasmid

#19423

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Tags

His

Expression

Bacterial

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pBK-CMV-mUBP43

Plasmid

#12459

Depositor

Dong-Er Zhang

Article

Liu et al Mol Cell Biol. 1999 Apr . 19(4):3029-38.

Insert

UBP43 (Usp18 Mouse)

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
mPlum-Gamma-Tubulin-17

Plasmid

#55971

Purpose

Localization: Centrosomes, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
GAL4-mNFAT1(2-144)

Plasmid

#11108

Purpose

Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells

Depositor

Anjana Rao

Article

Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

Insert

NFAT1 (Nfatc2 Mouse)

Tags

GAL4-DBD

Expression

Mammalian

Mutation

Contains NFAT1 amino acids 2-144; L78P and P140Q …

Available Since

April 24, 2006

Availability

Academic Institutions and Nonprofits only

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