We narrowed to 2,204 results for: CO

1. pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

   Plasmid

   #223155

   Purpose

   Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD2 (MBD2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 176-231

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pToxAmp12

   Plasmid

   #218286

   Purpose

   Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-N-Blast-moxGFP-LMNB1

   Plasmid

   #207772

   Purpose

   Donor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-moxGFP Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. LifeAct-mNeonGreen -IRES- Talin head-SpyTag003

   Plasmid

   #210024

   Purpose

   Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   LifeAct-mNeonGreen co-expressed with Talin head(1-433)-SpyTag003
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Encephalomyocarditis virus internal ribosome entr…

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Blast-miniTurbo-LMNB1

   Plasmid

   #207774

   Purpose

   Donor template for Blast-2A-miniTurbo insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-miniTurbo Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. qTAG-N-Blast-V5-LMNB1

   Plasmid

   #207779

   Purpose

   Donor template for Blast-2A-V5 insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-V5 Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. qTAG-C-Solo-mScarlet-H3C2

   Plasmid

   #207781

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a mScarlet Tag (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. qTAG-C-mNeon-Blast-H3C2

   Plasmid

   #207782

   Purpose

   Donor template for mNeon-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a mNeon-Blast Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Solo-mScarlet-ACTB

   Plasmid

   #207750

   Purpose

   Donor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. qTAG-N-Solo-mNeon-ACTB

    Plasmid

    #207749

    Purpose

    Donor template for mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a mNeon Tag (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-N-Blast-moxGFP-TUBA1B

    Plasmid

    #207766

    Purpose

    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Blast-moxGFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pTS2326

    Plasmid

    #169628

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTS2321

    Plasmid

    #169626

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTS2329

    Plasmid

    #169629

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTS2322

    Plasmid

    #169627

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and Tactb-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTS2320

    Plasmid

    #169625

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS2312

    Plasmid

    #169622

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTS2317

    Plasmid

    #169624

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)-GFP11-RhoA

    Plasmid

    #181860

    Purpose

    Part of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    GFP11-RhoA (RHOA Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAB(EXPR-HDAC5-trun1)

    Plasmid

    #114400

    Purpose

    For PYL-HDAC5 aa 1-275 expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5 aa 1-275 (HDAC5 Human)
    

    Use

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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