We narrowed to 4,657 results for: IRES

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pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro

Plasmid

#185451

Purpose

Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells

Depositor

Ahmet Yildiz

Article

Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

Insert

Nucleocapsid (N SARS-CoV-2)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-rtTA-IRES-GFP-WPRE-pA

Plasmid

#183709

Purpose

AAV designed to express rtTA and eGFP from a CMV promoter

Depositor

Jonathan Ploski

Insert

rtTA

Use

AAV

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIPdU3-MND-FLAG-FLI1-IRES-GFP

Plasmid

#129496

Purpose

Lentiviral expression of human FLI1

Depositor

Marjorie Brand

Article

Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

Insert

FLI1 (FLI1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

MND

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

Plasmid

#113890

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

Plasmid

#75126

Purpose

Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Mutation

SET domain replaced with the SET domain of EZH1

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pLVX-Flag-CDK19-W105M-IRES-ZsGreen

Plasmid

#68859

Purpose

Expresses Human CDK19 W105M Mutant

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 19 (CDK19 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

W105M

Promoter

EF1α

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-SLC7A11-sh926R-FLAG-IRES-Hygro

Plasmid

#118702

Purpose

lentiviral vector experessing SLC7A11 insensitive to hairpin TRCN0000288926

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

SLC7A11 (SLC7A11 Human)

Use

Lentiviral

Tags

Flag

Mutation

wt , SLC7A11 sh926R

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMIR-DsRed-IRES-His-Halo-Tev-Keap1

Plasmid

#58240

Purpose

expresses halo tagged human Keap1 protein

Depositor

Yimon Aye

Article

Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

Insert

Kelch like ECH associated protein 1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only
Dnase1L3 R206C pIRES-DsRed

Plasmid

#98847

Purpose

Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed

Depositor

Peter Keyel

Article

Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

Insert

Dnase1L3 (DNASE1L3 Human)

Tags

IRES DsRed

Expression

Mammalian

Mutation

R206C which greatly reduces nuclease activity

Promoter

CMV

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBEG R2-eGFP-L3 #BGV201

Plasmid

#48979

Purpose

Supplies the selection marker (eGFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Insert

eGFP

Use

Gateway Cloning

Tags

IRES (strong)

Promoter

NA

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pBEG R2-iPuro-L3 #BGV205

Plasmid

#48987

Purpose

Supplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Insert

puromycin resistance

Use

Gateway Cloning

Tags

IRES (weak)

Promoter

NA

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S

Plasmid

#62458

Purpose

expresses his-tagged C151S human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteine 151 of Keap1 to serine

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD

Plasmid

#169818

Purpose

Expresses C-terminal flag-tagged CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCCC -> AGTC silent mutations at nt527-530 eli…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-SADB19_oG-IRES-Puro

Plasmid

#172367

Purpose

Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

oG + Puro

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)

Plasmid

#139448

Purpose

Lentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag E2-crimson

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1α-IRES-EGFP-hMCIDAS

Plasmid

#149696

Purpose

Expresses untagged human MCIDAS in mammalian cells

Depositor

Ken-Ichi Takemaru

Insert

MCIDAS (MCIDAS Human)

Use

Lentiviral

Promoter

EF1 alpha

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Caspase-1 (C285A)

Plasmid

#131353

Purpose

Expresses Caspase-1 C285A

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

Caspase-1 (C285A) (Casp1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Caspase-1 (C285A)

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-NRASQ61K-IRES-Puro

Plasmid

#120575

Purpose

Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV mouse Vav1 IRES GFP

Plasmid

#107091

Purpose

Mammalian expression retroviral vector

Depositor

Steven Clifford, Martine Roussel

Article

Lindsey et al Oncogene. 2015 Sep 3;34(36):4746-57. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1a-RoninDHSA-FLAG-IRES-Neo

Plasmid

#28021

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Insert

Ronin (THAP11 Human)

Tags

FLAG

Expression

Mammalian

Mutation

DHSA mutation (Y246A) of the previously defined H…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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