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We narrowed to 8,696 results for: crispr c plasmids

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Showing: 1821 - 1840 of 8696 results

  1. (942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195624
    Purpose
    pAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Use
    AAV
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195624-plasmid-map-sequence-id-385116

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  2. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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  9. PB-PE SpRY

    Plasmid
    #179082
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…
    Promoter
    CAG
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179082-plasmid-map-sequence-id-352656

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  11. PB-PE SpG

    Plasmid
    #179081
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R
    Promoter
    CAG
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179081-plasmid-map-sequence-id-352652

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  15. pZNF57-donor

    Plasmid
    #112296
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF57 with GFP
    Depositor
    Kevin White
    Insert
    ZNF57 homology arms flanking EGFP-IRES-Neo cassette (ZNF57 Human)
    Use
    CRISPR
    Mutation
    rs2288959, rs2288958, rs10410539, hg38:19:2918211…
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112296-plasmid-map-sequence-id-225584

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  16. pZNF565-donor

    Plasmid
    #112315
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF565 with GFP
    Depositor
    Kevin White
    Insert
    ZNF565 homology arms flanking EGFP-IRES-Neo cassette (ZNF565 Human)
    Use
    CRISPR
    Mutation
    hg38:19:36182553:A>T, hg38:19:36182595:A>G,…
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112315-plasmid-map-sequence-id-228476

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  17. pNR1D2-donor

    Plasmid
    #112314
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR1D2 with GFP
    Depositor
    Kevin White
    Insert
    NR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
    Use
    CRISPR
    Mutation
    hg38:3:23977483:A>T, hg38:3:23977515:A>G, h…
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112314-plasmid-map-sequence-id-228473

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  18. pSMAD3-donor

    Plasmid
    #112311
    Purpose
    CRISPR donor plasmid to tag human transcription factor SMAD3 with GFP
    Depositor
    Kevin White
    Insert
    SMAD3 homology arms flanking EGFP-IRES-Neo cassette (SMAD3 Human)
    Use
    CRISPR
    Mutation
    hg38:15:67189762:C>T, rs11629568, rs8025774
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112311-plasmid-map-sequence-id-228463

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  19. pCas

    Plasmid
    #62225
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgR
    Depositor
    Sheng Yang
    Article
    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii
    Has Service
    Cloning Grade DNA
    Insert
    cas9
    Use
    CRISPR; Lambda red recombinase
    Expression
    Bacterial
    Promoter
    native cas9 promoter
    Available Since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    62225-plasmid-map-sequence-id-275267

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  20. pCRIS

    Plasmid
    #120424
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.
    Depositor
    Tamás Fehér
    Article
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3, IS150
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    constitutive
    Available Since
    Jan. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    120424-plasmid-map-sequence-id-233246

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Showing: 1821 - 1840 of 8696 results