We narrowed to 7,632 results for: ef1a
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Plasmid#250269PurposeInducible expression of FBXO31(D334N) with the shield degron system.DepositorInsertFBXO31 (FBXO31 Human)
UseLentiviralTagsDD-3xFLAGExpressionMammalianMutationD334NPromoterEF-1aAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
LSM0252 (PB)EF1a-FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP
Plasmid#233443PurposePiggyBac donor plasmid to overexpress FKBP-ADAR2dd (cLIDAR)DepositorInsertFKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP
ExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceJune 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP
Plasmid#233446PurposePiggyBac donor plasmid to overexpress ADAR2dd-PCPDepositorInsertADAR2dd-PCP-T2A-PuroR-P2A-BFP
ExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP
Plasmid#233441PurposePiggyBac donor plasmid to overexpress CCR6-ADAR2dd (gLIDAR)DepositorInsertCCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP
ExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP
Plasmid#233444PurposePiggyBac donor plasmid to overexpress ADAR2dd-MCPDepositorInsertADAR2dd-MCP-T2A-PuroR-P2A-BFP
ExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA
Plasmid#233046PurposeTo express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.DepositorInsertRfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
TagsmCherryExpressionMammalianAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA
Plasmid#233045PurposeTo express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.DepositorInsertRfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
TagsmCherryExpressionMammalianAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export
Plasmid#233382PurposeOptimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequenceDepositorInsertVEGFR1(ecto+TM)-ADAR2dd
TagsFLAGExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)
Plasmid#229742PurposeExpresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.DepositorInsertLRRK2 (LRRK2 Human)
TagsHaloExpressionMammalianMutationLIR1 mutant WEVL -> AEVAPromoterEF1AAvailable SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)
Plasmid#229743PurposeExpresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.DepositorInsertLRRK2 (LRRK2 Human)
TagsHaloExpressionMammalianMutationLIR2 mutant WTFI -> ATFAPromoterEF1AAvailable SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA
Plasmid#184636PurposeExpresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGat1 (Slc6a1 Mouse)
UseAAVMutationGat1 gene with Arginine 69 changed to Lysine, to …PromoterEF1aAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)
Plasmid#171099PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.DepositorAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2
Plasmid#118159PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3
Plasmid#118160PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
Plasmid#20297PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activationDepositorHas ServiceAAV Retrograde, AAV1, AAV5, AAV8, and AAV9Insertchannelrhodopsin-2
UseAAVTagsmCherryExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable SinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGIPZ EF1a myc tagged mbIL2 IRES miRFP720-P2A-Blast
Plasmid#248031PurposeConstitutively expresses myc tagged membrane bound IL2, miRFP720 and a blasticidin resistance gene in human cellsDepositorInsertmyc-tagged membrane bound IL2 (IL2 Human)
UseLentiviralExpressionMammalianPromoterhuman EF1aAvailable SinceJan. 16, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro
Plasmid#31482DepositorInsertTraffic Light Reporter 1.1 (Sce Target) EF Puro
UseLentiviralMutationmCherry has M9S and M16L mutations. This is what …Available SinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP
Plasmid#232800PurposeHelper plasmid to trigger auxin-inducible-degradation of targeted proteins.DepositorInsertOsTIR1 F74G EGFP-AID
UseLentiviralAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)
Plasmid#217306PurposesgRNA mcherry + PuromycinDepositorInsertpU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
UseCRISPRPromoterU6, Ef1alphaAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP
Plasmid#31481DepositorInsertTraffic Light Reporter 1.1 (Sce target) EF BFP
UseLentiviralMutationmCherry contains M9S and M16L to reduce backgroun…Available SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only