We narrowed to 15,550 results for: pET

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pET22b-T7-6h-GST-α-Synuclein

Plasmid

#225224

Purpose

Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

Depositor

Urartu Seker

Article

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

GST
α-Synuclein

Tags

6xHis Tag and TEV Cleavage Site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-Cas9

Plasmid

#242759

Purpose

Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Article

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-Cas9

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-15

Plasmid

#228971

Purpose

For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-15

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET11a-p65(19-325)

Plasmid

#44744

Depositor

Gourisankar Ghosh

Insert

p65 (Rela Mouse)

Expression

Bacterial

Mutation

AA 19-325

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-HaloTag7-SnoopLigase

Plasmid

#105627

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

HaloTag7-SnoopLigase

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaBC (pCLK1210)

Plasmid

#50448

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

Insert

PPCS PPCDC

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp3-His

Plasmid

#11821

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-sfGFP

Plasmid

#199171

Purpose

used to express a superfolder GFP in E. coli for comparison to supercharged variants

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

superfolder GFP

Tags

MGHHHHHHGGA

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pETcon_SARS-CoV-2_RBD

Plasmid

#166782

Purpose

Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

Depositor

Jesse Bloom

Article

Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

Insert

Spike receptor binding domain (RBD) from SARS-CoV-2 (S )

Expression

Yeast

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-p52(1-398)

Plasmid

#44733

Depositor

Gourisankar Ghosh

Insert

p52 (NFKB2 Human)

Expression

Bacterial

Mutation

AA 1-398

Promoter

T7

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pET-28c(+)-YTHDF2

Plasmid

#102275

Purpose

For bacterial expression of His-tagged human YTHDF2

Depositor

Samie Jaffrey

Article

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDF2 (YTHDF2 Human)

Tags

6X HIS

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDBamHI-Leu

Plasmid

#38256

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pET-MBP-Geo_st

Plasmid

#87700

Purpose

Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

Depositor

Jennifer Doudna

Insert

GeoCas9

Tags

10xHis-MBP-TEV

Expression

Bacterial

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET-11-LECT2-I40

Plasmid

#183032

Purpose

Expression of LECT2 I40 variant protein from T7 promoter

Depositor

Gareth Morgan

Article

Belonogov et al bioRxiv 2022.03.01.482540

Insert

LECT2 (LECT2 Human)

Expression

Bacterial

Mutation

I40 variant

Promoter

T7

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET21 gamma-tubulin

Plasmid

#101825

Purpose

production of gamma-tubulin in bacteria

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

His

Expression

Bacterial

Mutation

Whole protein

Promoter

T7

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-hTBP

Plasmid

#171073

Purpose

Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

TATA-box-binding protein (TBP Human)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16

Plasmid

#172746

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(41-140)

Plasmid

#240596

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 41-140 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(36-140)

Plasmid

#240595

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 36-140 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp9-His

Plasmid

#11829

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

Insert

Caspase 9 (CASP9 Human)

Tags

6xHis

Expression

Bacterial

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only