We narrowed to 7,809 results for: pcDNA 3.1

1. pcDNA3.1-PDGFRA-K627M-V5/HIS

   Plasmid

   #234755

   Purpose

   Expression of the K627M kinase defective mutant variant of human PDGFRA

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   PDGFRA-K627M receptor tyrosine kinase, full length (PDGFRA Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   K627M substitution

   Promoter

   CMV

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3

   Plasmid

   #233062

   Purpose

   Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Tags

   Alfa and HiBiT

   Expression

   Mammalian

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt

   Plasmid

   #233240

   Purpose

   Parallel coiled-coil with (GGS)3 linker to prone condensation

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-clover-GCN4-(GGS)3-Vt
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt

   Plasmid

   #233241

   Purpose

   Parallel coiled-coil with (GGS)12 linker to prevent condensation

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-clover-GCN4-(GGS)12-Vt
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 FFSS-BACH1 C122E

   Plasmid

   #232274

   Purpose

   Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell lines

   Depositor

   Michele Pagano

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   BACH1 (BACH1 Human)
   

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   changed cysteine 122 to glutamic acid

   Promoter

   CMV

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Flag-Jarid2-polyA

   Plasmid

   #223811

   Purpose

   IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

   Depositor

   Azusa Inoue

   Article

   Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

   Insert

   Jarid2 (Jarid2 Mouse)
   

   Use

   In vitro transcription

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(-)-EGFP-AP4-cyto

   Plasmid

   #229694

   Purpose

   Transient expression of EGFP AP4-cyto in mammalian cells

   Depositor

   Alpha Yap

   Article

   Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

   Insert

   EGFP; Control construct with tandem AP4 motifs (cytoplasm)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(-)-EGFP-FP4-cyto

   Plasmid

   #229695

   Purpose

   Transient expression of FP4-cyto in mammalian cells

   Depositor

   Alpha Yap

   Article

   Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

   Insert

   EGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-myc:FRB:gs10:Cul1(dC501)

   Plasmid

   #228533

   Purpose

   A fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   myc:FRB:gs10:Cul1(dC501) (CUL1 Human, Synthetic)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-myc:Rbx1:gs10:FRB:HA

    Plasmid

    #228534

    Purpose

    Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:Rbx1:gs10:FRB:HA (RBX1 Human, Synthetic)
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA 3.1-NbON10-NanoLuc

    Plasmid

    #198691

    Purpose

    Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    

    Tags

    6xHis tag, IgK leader sequence, NanoLuc, and twin…

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(-).TagRFP.T-2A-Tau2N4R-WPRE

    Plasmid

    #226380

    Purpose

    Expresses tau propagation ORF with no V5 tag on WT 2N4R tau

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT synthetic, Human)
    

    Tags

    TagRFP.T-2A

    Expression

    Mammalian

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(-).TagRFP.T-2A-V5-8D2Q.Tau2N4R-WPRE

    Plasmid

    #226382

    Purpose

    Expresses tau propagation ORF with V5 tag on 8D2Q mutant 2N4R tau

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT synthetic, Human)
    

    Tags

    TagRFP.T.2A.V5

    Expression

    Mammalian

    Mutation

    8D2Q.Tau2N4R

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(-).TagRFP.T-2A-V5-20D3Q.Tau2N4R-WPRE

    Plasmid

    #226383

    Purpose

    Expresses tau propagation ORF with V5 tag on 20D3Q mutant 2N4R tau

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT synthetic, Human)
    

    Tags

    TagRFP.T.2A.V5

    Expression

    Mammalian

    Mutation

    20D3Q, Tau2N4R

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(-).TagRFP.T-2A-V5-8A2R.Tau2N4R-WPRE

    Plasmid

    #226384

    Purpose

    Expresses tau propagation ORF with V5 tag on 8A2R mutant 2N4R tau

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT synthetic, Human)
    

    Tags

    TagRFP.T.2A.V5

    Expression

    Mammalian

    Mutation

    8A2R, Tau2N4R

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(-).TagRFP.T-2A-V5-20A3R.Tau2N4R-WPRE

    Plasmid

    #226385

    Purpose

    Expresses tau propagation ORF with V5 tag on 20A3R mutant 2N4R tau

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT synthetic, Human)
    

    Tags

    TagRFP.T.2A.V5

    Expression

    Mammalian

    Mutation

    20A3R, Tau2N4R

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(-).TagRFP.T-2A-V5-K281Q.Tau2N4R-WPRE

    Plasmid

    #226386

    Purpose

    Expresses tau propagation ORF with V5 tag on K281Q mutant 2N4R tau

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT synthetic, Human)
    

    Tags

    TagRFP.T.2A.V5

    Expression

    Mammalian

    Mutation

    K281Q, 2N4R

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(-).TagRFP.T-2A-V5-K353Q.Tau2N4R-WPRE

    Plasmid

    #226387

    Purpose

    Expresses tau propagation ORF with V5 tag on K353Q mutant 2N4R tau

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT synthetic, Human)
    

    Tags

    TagRFP.T.2A.V5

    Expression

    Mammalian

    Mutation

    K353Q, 2N4R

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(-).TagRFP.T-2A-V5-S198D.Tau2N4R-WPRE

    Plasmid

    #226388

    Purpose

    Expresses tau propagation ORF with V5 tag on S189D mutant 2N4R tau

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT synthetic, Human)
    

    Tags

    TagRFP.T.2A.V5

    Expression

    Mammalian

    Mutation

    S198D.Tau2N4R

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(-).TagRFP.T-2A-V5-S199D.Tau2N4R-WPRE

    Plasmid

    #226389

    Purpose

    Expresses tau propagation ORF with V5 tag on S199D mutant 2N4R tau

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT synthetic, Human)
    

    Tags

    TagRFP.T-2A-V5

    Expression

    Mammalian

    Mutation

    S199D, Tau2N4R

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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