We narrowed to 4,802 results for: pcr
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GPR84-Tango
Plasmid#66377PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZC13 (pFA6a-TEV-6xGly-V5-HphMX)
Plasmid#44085DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, HphM…Available SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF279 (pFA6a-TEV-6xGly-2xStrep-HphMX)
Plasmid#44082DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…Available SinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)
Plasmid#44075DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…Available SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF269 (pFA6a-PSc-6xGly-12xHis-KanMX)
Plasmid#44087DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…Available SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF278 (pFA6a-PSc-6xGly-2xStrep-KanMX)
Plasmid#44088DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…Available SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔC
Bacterial Strain#102258PurposeompC deletion strain for overproduction of outer membrane proteinsDepositorBacterial ResistanceNoneAvailable SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX1-Tango
Plasmid#66439PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
CYSLTR2-Tango
Plasmid#66267PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-gamma-5 in pcDNAI/Amp
Plasmid#55625PurposeAn amino-terminal mCerulean fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCer(1-158)-gamma-5 (GNG5 Aequorea victoria, Human)
TagsCer(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-5 was amplified by PCR, which added a BamH…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔAC
Bacterial Strain#102261PurposeompA, ompC double knockout strain for overproduction of outer membrane proteinsDepositorBacterial ResistanceNoneAvailable SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔAF
Bacterial Strain#102262PurposeompA, ompF double knockout strain for overproduction of outer membrane proteinsDepositorBacterial ResistanceNoneAvailable SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔABF
Bacterial Strain#102267PurposeompA, lamB, ompF triple knockout strain for overproduction of outer membrane proteinsDepositorBacterial ResistanceNoneAvailable SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
p12x601
Plasmid#157785PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backboneDepositorInsertpMD2.G(spei/spei)&12x601_tetO
ExpressionBacterialAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1328
Plasmid#69488PurposesgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegansDepositorInsertsgRNA(F+E) targeting pha-1
ExpressionWormPromoterR07E5.16 U6 promoterAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBALU9
Plasmid#69324PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOPDepositorInsertgSL2-NLS-GFPint-FgF
ExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238)-beta-5 in pcDNAI/Amp
Plasmid#55763PurposeA carboxyl-terminal CFP fragment was fused to Gbeta-5. When co-expressed with an amino terminal CFP or YFP fragment fused to a Ggamma subunit or RGS7, a fluorescent signal is produced.DepositorInsertCFP(159-238)-beta-5 (GNB5 Aequorea victoria, Human)
TagsCFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationCFP(159-238) includes a substitution of His for A…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-beta-2 in pcDNAI/Amp
Plasmid#54469PurposeAn amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertYFP(1-158)/beta-2 (GNB2 Aequorea victoria, Human)
TagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationYFP (1-158) includes a substitution of Met for Gl…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLO104
Plasmid#90224PurposeComplements mutations in Aspergillus nidulans gene riboB (AN0670) (mutants require riboflavin)DepositorInsertATET_00531
UseAspergillusPromoterpLacAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only
