We narrowed to 4,839 results for: pcr
-
Plasmid#171048PurposeEmpty donor template for CRISPR/Cas9 targeting of TERT RE'sDepositorTypeEmpty backboneUseCre/LoxExpressionMammalianAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
pKM343
Plasmid#71487PurposeContains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.DepositorInsertloxP-hyg-cat-loxP
UseSource of loxp-hyg-cat- loxp for pcr amplificatio…ExpressionBacterialAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔBCF
Bacterial strain#102269PurposelamB, ompC, ompF triple knockout strain for overproduction of outer membrane proteinsDepositorBacterial resistanceNoneAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYZ038
Plasmid#200227Purposeuniversal template to build tRNA-gRNA array for multiplex editing CREEPYDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttRNA-gRNA scaffold
UseSynthetic BiologyAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
ML6
Bacterial strain#61908PurposeC43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. ilvE, avtA genes deleted.DepositorBacterial resistanceKanamycinAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔA
Bacterial strain#102256PurposeompA deletion strain for overproduction of outer membrane proteinsDepositorBacterial resistanceNoneAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
delta-ZYA
Bacterial strain#52695PurposeMG1655 with Lac Operon removed from native locusDepositorBacterial resistanceNoneAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSHA-ARF4-Myc
Plasmid#67350PurposeMammalian expression of hArf4 with C-term Myc tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertARF4 (ARF4 Human)
TagsMycExpressionMammalianMutationV53A, L123P and M134I mutations were unintended P…PromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
ML2
Bacterial strain#61906PurposeC43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. ilvE gene deleted.DepositorBacterial resistanceKanamycinAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBALU1
Plasmid#69316PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfpDepositorInsertGFPint-FgF
ExpressionBacterialAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-HBTH
Plasmid#26898DepositorInsertHBTH tag N-terminal
UsePcr tagging yeastAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-RGS7
Plasmid#55760PurposeAn amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
TagsCer(1-158) was fused to the amino terminus of RGS…ExpressionMammalianMutationRGS7 was amplified via PCR, which added an N-term…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAG32-3HA-3FLAG
Plasmid#154081PurposeFor C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.DepositorInsert3xHA-3xFLAG-hygR
UsePcr-based tagging in yeastTags3xHA-3xFLAGAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ML21
Bacterial strain#61914PurposeC43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG tyrA genes deleted.DepositorBacterial resistanceNoneAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCY 3040-01
Plasmid#36217DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertyEGFP
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEGFP-kanMXAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
JEC027
Bacterial strain#180310PurposeE. coli MG1655 strain with knockout of endogenous Type I-E CRISPR Cas systemDepositorBacterial resistanceNoneAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR26-CMVconst-mBMP7
Plasmid#127375PurposeAllows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBMP7 (Bmp7 Mouse)
UseCRISPR and Mouse TargetingPromoterCMV enhancer/promoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRNA-hIRF-1 #12
Plasmid#61079PurposeExpresses a guide RNA (gRNA) to target human IRF-1 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA_hIRF1 promoter #12 (IRF1 Human)
UseCRISPRExpressionMammalianPromoterU6Available sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKnockinDonor-humanCTCF-miniAID-mClover3
Plasmid#136883Purposeknockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCFDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTCF-knockin-homology arm-miniAID-mClover3
TagsminiAID-mClover3ExpressionMammalianPromoterno promoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-AID*(N)
Plasmid#99525PurposeN-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only