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gfp-Arkadia/ RNF111 deltaRING

Plasmid

#112229

Purpose

Expresses GFP-tagged enzymatically inactive (delta RING) Arkadia in mammalian cells

Depositor

Vasso Episkopou

Article

Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

Insert

(Arkadia)Rnf111 (Rnf111 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

delta RING domain deletion (Deletion of the last …

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only
pGEX Socs4-SH2

Plasmid

#46507

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs4 (SOCS6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 376-498

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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hMEF2A K403R

Plasmid

#118355

Purpose

This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K403R

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX tensin-SH2

Plasmid

#46525

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

tensin (TNS1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1451-1588

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Zap70(N)-SH2

Plasmid

#46534

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Zap70(N) (ZAP70 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 1-116

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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D998-EGFR-ET1

Plasmid

#44186

Depositor

Timothy Springer

Article

Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

Insert

D998-EGFR-ET1 (EGFR Human)

Tags

3C cleavge site, protein C peptide tag, his6, SBP…

Expression

Mammalian

Mutation

EGFR amino acids 1−998

Promoter

CVM

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 D444-500

Plasmid

#19839

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 D444-500 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 444-500

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pGEX Jak2-SH2

Plasmid

#46448

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Jak2 (JAK2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 394-497

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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DF-UAS-spa-GCaMP6f

Plasmid

#67568

Purpose

Zebrafish expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Use

Zebrafish transgenesis (see :a spinal opsin contr…

Tags

HIS-tag

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 T450A

Plasmid

#19843

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 T450A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Threonine 450 to Alanine

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pGEX Grb14-SH2

Plasmid

#46439

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb14 (GRB14 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 427-540

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pBT259_(pRosa26-G-tTA2)

Plasmid

#36878

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
diphteria toxin A

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pFIN-mIRBP156-CHER-RK-GFP-WPRE

Plasmid

#44593

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Inserts

IRBP156-CHER
RK-GFP

Use

Lentiviral

Promoter

human rhodopsin kinase (pos 3723-4019) and murine…

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 D500-630

Plasmid

#19841

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 D500-630 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 500-630

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
p3xFLAG-MKL1 D381-506

Plasmid

#19853

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 381 to 506

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA6-E2F4CA-Myc

Plasmid

#80428

Purpose

Mammalian expression of constitutively active chick E2F4

Depositor

José María Frade

Article

Morillo et al Mol Cell Biol. 2012 Jul;32(14):2722-37.

Insert

E2F transcription factor 4 (E2F4 Chicken)

Tags

Myc

Expression

Mammalian

Mutation

T261E, T263E

Promoter

CMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRb delta CDK + S230

Plasmid

#58907

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S230 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T252

Plasmid

#58910

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T252 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pMIR-31-Reporter

Plasmid

#71871

Purpose

Mammalian expression vector for the analysis of miR-31 activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-31

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-CNPsgRNA

Plasmid

#126610

Purpose

Expresses CNP sgRNA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP sgRNA (Cnp Mouse)

Use

CRISPR

Promoter

mouse U6 promoter

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D

Plasmid

#32457

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S129D, S133D, S137D (S3V, G96R, and P108L also pr…

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-CaMKKbeta S129A, S133A, S137A

Plasmid

#32454

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

S129A, S133A, S137A

Promoter

CMV

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
pMA3431

Plasmid

#46876

Purpose

A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3 mutant R592Q

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1alpha 3 (Gucy1a3 Rat)

Use

Lentiviral

Tags

Myc

Mutation

R592Q

Promoter

TRE-Tight

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAC4 Grb14-PIRSH2

Plasmid

#46438

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb14 (GRB14 Human)

Tags

AviTag, GST, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 341-540

Promoter

Trc

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha218-1374

Plasmid

#62523

Purpose

N-terminal aa1-217 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-217 deleted (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-217

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
ADAP1

Plasmid

#163905

Purpose

Full length mouse ADAP1 for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha270-1374

Plasmid

#62524

Purpose

N-terminal aa1-269 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-269 deleted (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
pRevTRE SGFP2 Inpp5E WT shRNA resistant

Plasmid

#161997

Purpose

Tet-responsive retroviral expression vector. Contains INPP5E. Also contains SGFP tag.

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP5E (INPP5E Human)

Use

Retroviral

Tags

SGFP

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX Grb7-PIR-SH2

Plasmid

#46443

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb7 (GRB7 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 334-532

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX HSH2-SH2

Plasmid

#46446

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

HSH2 (HSH2D Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-134

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge