We narrowed to 2,241 results for: D10

1. R2pH-LAMP-FRB

   Plasmid

   #225138

   Purpose

   R2pH-LAMP1-labelled lysosomes with FRB

   Depositor

   Massimiliano Stagi

   Article

   Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

   Insert

   Lamp1 (Lamp1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmcherry-mTet1s pc3905

   Plasmid

   #201703

   Purpose

   Expresses of mcherry-tagged mTet1s in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1s (Tet1 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TH1534-DuET

   Plasmid

   #213246

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   TH1534 (FZD10 Human)
   

   Tags

   1D4 and H4

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNM1325

   Plasmid

   #197144

   Purpose

   CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Insert

   SpCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   SpCas9(D10A)

   Promoter

   CAG

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFlare9A-CD45i4e5i5mutCBS

   Plasmid

   #131835

   Purpose

   A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   PTPRC (PTPRC Human)
   

   Expression

   Mammalian

   Mutation

   partial intron 4 full exon 5 and partial intron 5…

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9

   Plasmid

   #163550

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-HF-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7pr-His6-MBP-TEV-Cas9-BirA*

   Plasmid

   #159993

   Purpose

   Bacterial expression of Cas9-BirA*

   Depositor

   Jacob Corn

   Article

   Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

   Insert

   Cas9-BirA*
   

   Tags

   6X-His, MBP, 3X-FLAG, NLS

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   T7

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJT175_GalL_A3A(Y130F)Δ186-BE3

   Plasmid

   #145124

   Purpose

   Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(Y130F)Δ186-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   A3A(Y130F; 1-186AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189922

   Purpose

   AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e
   

   Use

   AAV

   Mutation

   SaCas9 D10A

   Promoter

   EFS

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas12Max-ABE

    Plasmid

    #195335

    Purpose

    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized dCas12Max-ABE
    

    Expression

    Mammalian

    Mutation

    N243R, E336R, D1049A

    Promoter

    CBh, CMV, hU6

    Available Since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-CAGGS-dCas9-KRAB-MeCP2

    Plasmid

    #110824

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2T-CMV-ABE8e_dNRCH-BlastR

    Plasmid

    #232720

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    ABE8e-dCas9(NRCH)
    

    Expression

    Mammalian

    Mutation

    within Cas9 D10A H840A

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. L4802 VEGFR2 NTEVp chain (WT NTEVp) in PolyTX-mNeonGreen

    Plasmid

    #244173

    Purpose

    Constitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(WT)

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA NTEVp chain with human VEGFR2 SS, ECD and TMD, and NTEVp (WT) (KDR Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    VEGFR2 signal peptide - 3xFLAG

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-Solo-mStayGold-LAMP1

    Plasmid

    #227322

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid

    #84243

    Purpose

    Expresses ABA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. LAMP-mCherry-CRY2

    Plasmid

    #102249

    Purpose

    Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    LAMP-mCherry-CRY2 (LAMP1, CRY2 Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid

    #73499

    Purpose

    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-OptoSTIM1

    Plasmid

    #70159

    Purpose

    Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1 (STIM1 Mustard Weed, Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tet1-dCas9

    Plasmid

    #136650

    Purpose

    Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Tet1-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-KIF5A

    Plasmid

    #166958

    Purpose

    Expresses HA-tagged KIF5A protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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