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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,493 results for: D10

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Showing: 1861 - 1880 of 2493 results

  4. pSCBE3-NG

    Plasmid
    #218159
    Purpose
    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / L1111…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218159-plasmid-map-sequence-id-433199

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  5. 3xFLAG-dCas9/pMSCVpuro

    Plasmid
    #134983
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134983-plasmid-map-sequence-id-430623

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  6. AID-C12*-BE4max

    Plasmid
    #216729
    Purpose
    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.
    Depositor
    Rahul Kohli
    Publication
    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AID-C12*-BE4max (AICDA Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    216729-plasmid-map-sequence-id-429719

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  7. sABE v3.22 Lenti_1

    Plasmid
    #209555
    Purpose
    pCMV-SV40NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN
    Depositor
    Xue Gao
    Publication
    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN
    Expression
    Mammalian
    Promoter
    pCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    209555-plasmid-map-sequence-id-417946

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  9. TFORF3262

    Plasmid
    #144738
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CEBPE (CEBPE Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144738-plasmid-map-sequence-id-404340

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  13. pMEH60 Lamp2b MS2 HA

    Plasmid
    #168209
    Purpose
    Mammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tag
    Depositor
    Joshua Leonard
    Publication
    Hung et al J Extracell Vesicles. 2016 May 13;5:3102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    HA and MS2 dimer
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    168209-plasmid-map-sequence-id-350168

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Showing: 1861 - 1880 of 2493 results