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pJS70

Plasmid

#207586

Purpose

GFP-TolA H22A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA H22A

Expression

Bacterial

Mutation

H22A

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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ChlorON-2

Plasmid

#193478

Purpose

Turn-on fluorescent sensor for chloride

Depositor

Sheel Dodani

Article

Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

Insert

ChlorON-2

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(ATP1)/pcDNA3

Plasmid

#51992

Purpose

mammalian expression of a Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
SPOON/ pcDNA3

Plasmid

#124840

Purpose

mammalian expression of SPOON, a spontaneously blinking green fluorescent protein

Depositor

Takeharu Nagai

Article

Arai et al ACS Chem Biol. 2018 Aug 17;13(8):1938-19

Insert

SPOON

Tags

No tag

Expression

Mammalian

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)

Plasmid

#173455

Purpose

Contains mScarlet-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP709

Plasmid

#79986

Purpose

Monomeric near-infrared fluorescent protein miRFP709 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP709

Expression

Bacterial

Promoter

araB

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBbB19c-GFP

Plasmid

#126139

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NKB

Plasmid

#184609

Purpose

For expression of a fluorescent sensor for neurokinin B in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NKB

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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DGK alpha

Plasmid

#35404

Depositor

Robert Lefkowitz, Stephen Prescott

Article

Nelson et al Science. 2007 Feb 2;315(5812):663-6.

Insert

DGK alpha (DGKA Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-pnGFP

Plasmid

#64913

Purpose

Encodes a Fluorescent Reporter for Peroxynitrite in Mammalian Cells

Depositor

Huiwang Ai

Article

Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

Insert

pnGFP

Tags

6xHis

Expression

Mammalian

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

CMV

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pJ211_WT_low

Plasmid

#128848

Purpose

Low copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCO603

Plasmid

#139736

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnLuz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

Luz

Use

Luciferase; Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEutRwc

Plasmid

#216334

Purpose

An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

Depositor

Mark Styczynski

Article

McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

Inserts

EutR
Superfolder green fluorescent protein

Expression

Bacterial

Available Since

Dec. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-Blue_CaMBI

Plasmid

#124097

Purpose

Blue calcium-modulated bioluminescent indicator

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Blue CaMBI

Expression

Mammalian

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCO600

Plasmid

#139734

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnHisps

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

Hisps

Use

Luciferase; Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1.2

Plasmid

#113937

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1.2

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Green_CaMBI

Plasmid

#124096

Purpose

Green calcium-modulated bioluminescent indicator

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Green CaMBI

Expression

Mammalian

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT21.RY

Plasmid

#201006

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
sYFP2

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pL0M-S-tdTomato-EC18158

Plasmid

#137078

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

tdTomato DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-luxKOk

Plasmid

#229944

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKOk

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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