We narrowed to 5,570 results for: donor

1. TCF4 in pENTR

   Plasmid

   #216569

   Purpose

   gateway cloning donor vector containing TCF4

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Gateway entry plasmid

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCyRFP1-Cdc42

   Plasmid

   #84360

   Purpose

   fusion protein of Cdc42, FRET donor

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mCyRFP1-Cdc42
   

   Tags

   mCyRFP1 N terminal fusion to Cdc42

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA

   Plasmid

   #174028

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-eGFP
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AICSDP-72:TFAM-mEGFP

   Plasmid

   #159745

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TFAM

   Depositor

   Allen Institute for Cell Science

   Insert

   TFAM Homology Arms with linker-mEGFP (TFAM Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Oct. 13, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pTarget12f1

   Plasmid

   #171183

   Purpose

   Constitutive expression of sgRNA without donor DNA

   Depositor

   Kohsuke Honda

   Article

   Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

   Insert

   sgRNA scaffold in the CRISPR/Cas12f1 system
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119(SpeI) promoter

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHACK-GAL4>QF2(newHA1)

   Plasmid

   #104873

   Purpose

   HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   T2A-QF2
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA

   Plasmid

   #174030

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-Venus-PEST
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ204-loxP-100RPU.2-ETN-lox2272

   Plasmid

   #154833

   Purpose

   Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanercept

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Insert

   ETN
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   100RPU.2

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LT2_12_GlyRNA

   Plasmid

   #177710

   Purpose

   Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Inserts

   pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
   pTEFmut8-mCherry-tADH1
   

   Use

   Used as donor dna in genome integration after lin…

   Expression

   Yeast

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKHR7

    Plasmid

    #74624

    Purpose

    a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Insert

    cmlc2::mCherry
    

    Use

    Zebrafish expression

    Promoter

    cmlc2

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-TgKI-MCS-p2A-tdTomato-polyA

    Plasmid

    #174027

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-tdTomato
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA

    Plasmid

    #232935

    Purpose

    Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    T2A-eGFP-SV40pA with Grb10 homology arms
    

    Use

    Mouse Targeting

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFETCh_ZBTB1

    Plasmid

    #128235

    Purpose

    Donor vector for 3' FLAG tag of human ZBTB1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB1 Homology arms
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC1812_pCR8-gCasRx-CUG (control gRNA)

    Plasmid

    #118645

    Purpose

    Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gCasRx-CUG
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-TgKI-MCS-mScarlet-MCS-polyA

    Plasmid

    #174023

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    mScarlet
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBA787

    Plasmid

    #186242

    Purpose

    soc-C HR Donor Vector (T4 phage editing)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    BBR1 backbone + T4 phage homology
    

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGT-Ah3

    Plasmid

    #122570

    Purpose

    MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    beta-lactamase/sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTopo pLox hPGK-Puro pA pLox

    Plasmid

    #171048

    Purpose

    Empty donor template for CRISPR/Cas9 targeting of TERT RE's

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. mUAVbla:tetR 5′

    Plasmid

    #149379

    Purpose

    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Insert

    tetA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBA789

    Plasmid

    #186244

    Purpose

    soc-F HR Donor Vector (T4 phage editing)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    BBR1 backbone + T4 phage homology
    

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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