We narrowed to 33,032 results for: multi
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Plasmid#115211PurposeExpresses PsmCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPsmCas13
TagsSUMOExpressionBacterialAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKD‐SepRS-EFSep-5x tRNASep (B40 OTS)
Plasmid#52054PurposeUsed in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)DepositorInsertsSepRS
EF-Sep
5x tRNA Sep
ExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtacAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-sgCXCR4-2
Plasmid#46917PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 geneDepositorInsertsUseCRISPR and LentiviralExpressionMammalianPromoterCMV and U6Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHelper_ShCAST
Plasmid#127922PurposeExpresses ShCAST and a crRNA targeting PSP1DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k
Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI152-pLenti-SiT-Cas12a
Plasmid#128405PurposeExpress both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseLentiviralExpressionMammalianAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2+8
Plasmid#34931DepositorPublicationTypeEmpty backboneUseSea urchin, zebrafish, xenopusExpressionMammalianPromoterSP6Available sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B
Plasmid#64221PurposeExpression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2ADepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherry-P2A-E1BExpressionMammalianPromoterCBh and U6Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HBV 1.3-mer X-null replicon
Plasmid#65461Purposecontaining 1.3 units of the X-null HBV genome (subtype ayw)DepositorInsert1.3 units of the X-null HBV genome
ExpressionMammalianAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEJS614_pTetR-P2A-BFPnls/sgNS
Plasmid#108650PurposeNon-specific Spy sgRNA under U6 promoterDepositorInsertsNon-specific sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianPromoterU6 and hPGKAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC5Kan-P2A
Plasmid#51814PurposeP2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single messageDepositorInsertP2A peptide (PTV1gp1 porcine teschovirus-1, Synthetic)
UseShuttle vector for insect expression vectorsMutationcodons altered for Drosophila and to eliminate re…Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MOS
Plasmid#64120PurposeA modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genesDepositorAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)
Plasmid#103866PurposedPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.DepositorInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLH-sgRNA1-2XMS2
Plasmid#75389PurposesgRNA1-2XMS2DepositorInsertsgRNA1-2XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SP-GFP-CD274-NU
Plasmid#121139PurposeMammalian expression of GFP tagged CD274DepositorAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmTurquoise2-T2A-Venus(L68V)
Plasmid#60494PurposeProduces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.DepositorInsertmTurquoise2-T2A-mVenus(L68V)
TagsT2A peptideExpressionMammalianPromoterCMVAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p5E-elavl3
Plasmid#75025PurposeCan be used to drive pan neuronal expression in zebrafish.DepositorPublicationInsertELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
Use5' entry vector for multisite gateway three …Promoterelavl3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato
Plasmid#113865PurposeTriple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.DepositorInsertsCMV-eGFP
TOP-iRFP
PTCH1-mTurquoise2
CBF-tdTomato
UseLentiviralExpressionMammalianAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DS-SPcas
Plasmid#48645PurposeBacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
tracrRNA precursor
UseCRISPRPromoterproC and tracrRNA promoterAvailable sinceDec. 2, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT077
Plasmid#137879PurposeDonor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFPDepositorInsertTRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
UseAAVExpressionMammalianAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-smURFP-IRES-eGFP
Plasmid#80343PurposeExpresses smURFP and eGFP in mammalian cells.DepositorInsertssmURFP
eGFP
ExpressionMammalianPromoterCMV and IRESAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by