We narrowed to 10,414 results for: 158

1. pCAGGS-Flag-hsDicer (D1320A/D1709A)

   Plasmid

   #41585

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D1320A/D1709A

   Promoter

   CMV

   Available since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

   Plasmid

   #172501

   Purpose

   Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 full locus with Golden gate acceptor site for spacer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. G1397 DddAtox-C–SaKKH-Cas9(D10A)

   Plasmid

   #157840

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5FRT PUR mGSK3A K148A

   Plasmid

   #35158

   Depositor

   Jim Woodgett

   Article

   Doble et al Dev Cell. 2007 Jun;12(6):957-71.

   Insert

   GSK-3α (Gsk3a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K148A (dominant negative)

   Promoter

   CMV

   Available since

   May 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pWP1_mHAL

   Plasmid

   #102318

   Purpose

   expression of mouse cDNA of HAL refractory to the guide in plasmid 4

   Depositor

   David Sabatini

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   Hal (Hal Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBLO ddd64.1

   Plasmid

   #123121

   Purpose

   E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

   Depositor

   Jennifer Doudna, Benjamin Oakes

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   CasX
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Mutated amino acids in catalytic triad to alanine…

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV hSyn FLEx-loxp hM4Di-mCherry

   Plasmid

   #161581

   Purpose

   Expression of hM4Di-mCherry in a cre-dependent fashion

   Depositor

   Robert Malenka, Kevin Beier

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   hM4Di-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   hSyn

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFGL1252_TetOFF(Hyg)

   Plasmid

   #118992

   Purpose

   TetOFF (promoter-less) cassette with Hygromycin selection

   Depositor

   Naweed Naqvi

   Article

   Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

   Inserts

   tTA+Tnos
   Hygromycin cassette
   Ttub+PtetO
   

   Use

   Fungal expression (in magnaporthe oryzae)

   Tags

   Expression

   Mutation

   Codon optimized for Magnaporthe oryzae

   Promoter

   Aspergillus nidulans TrpC promoter, Ustilago mayd…

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRESneo-Ago2

   Plasmid

   #215841

   Purpose

   Expression of AGO2

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-mCherry2-sgMUC4

    Plasmid

    #198399

    Purpose

    Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    MUC4 sgRNA (MUC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LightR-iCre-miRFP670

    Plasmid

    #162158

    Purpose

    Expresses LightR-iCre-miRFP670 in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    LightR-iCre-miRFP670
    

    Use

    Tags

    NLS and miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PBLO CasX-gRNA

    Plasmid

    #126419

    Purpose

    E. coli vector for CasX gRNA --- Used for In Vitro Transcription

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    casX gRNA
    

    Use

    CRISPR; In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINK-VCPIP (OTU, aa 25-561)

    Plasmid

    #61583

    Purpose

    Expresses human VCPIP (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    VCPIP (VCPIP1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-24 and aa 562-1222.

    Promoter

    T7

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG

    Plasmid

    #176400

    Purpose

    Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    FRB-RGG-GFP-RGG-RGG
    

    Use

    Tags

    FRB and GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-6P-2_dCrk

    Plasmid

    #131146

    Purpose

    Expresses GST::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172504

    Purpose

    Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQTEV-COPE

    Plasmid

    #31585

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    coatomer protein complex, subunit epsilon (COPE Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. PhyB-mCherry-Spc72

    Plasmid

    #51582

    Purpose

    Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    Spc72 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLPC-mCh-ACTB-P2A-EGFP

    Plasmid

    #165158

    Purpose

    Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

    Depositor

    Taekjip Ha

    Article

    Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

    Insert

    mCherry-ACTB-P2A-EGFP (ACTB Human)
    

    Use

    Retroviral

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. G1333 DddAtox-C–SaKKH-Cas9(D10A)

    Plasmid

    #157838

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge