We narrowed to 12,568 results for: 109

1. pUC-fFuCas9-HTBNLS-hph

   Plasmid

   #121092

   Purpose

   Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

   Depositor

   Xiao-Jun Ji

   Article

   Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

   Insert

   Cas9 and hygromycin resist gene
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   gpdA;trpC

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1α:flex-mcherry

   Plasmid

   #222618

   Purpose

   Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

   Depositor

   Franck Polleux

   Article

   Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

   Insert

   Cre-dependent mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV7.1_3xFLAG-TAOK2

   Plasmid

   #172992

   Purpose

   constitutive expression of FLAG-tagged TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-TAOK2 (TAOK2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT003

   Plasmid

   #171628

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and EF1-a

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV7.1_3xFLAG-TAOK1

   Plasmid

   #172991

   Purpose

   constitutive expression of FLAG-tagged TAOK1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-TAOK1 (TAOK1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCherry-Tubulin-C-18

   Plasmid

   #55148

   Purpose

   Localization: Microtubules, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-GFP-WT1 +KTS N terminal HA

   Plasmid

   #217639

   Purpose

   Entry vector with WT1 +KTS mutant with N terminal HA for gateway cloning

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 +KTS isoform (WT1 Human)
   

   Use

   Gateway cloning

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-GFP-WT1 -KTS N terminal HA

   Plasmid

   #217641

   Purpose

   Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 -KTS isoform (WT1 Human)
   

   Use

   Gateway cloning

   Tags

   HA

   Expression

   Mutation

   p.408_410delKTS

   Promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJRH051

   Plasmid

   #171625

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BsmBI-Destination
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmTurquoise2-LC3

    Plasmid

    #110947

    Purpose

    Expresses the autophagosome marker LC3 fused to mTurquoise2

    Depositor

    Pablo Rivera-Fuentes

    Article

    Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_18xTEAD_luc2

    Plasmid

    #173003

    Purpose

    firefly luciferase reporter vector with 18x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    18xTEAD-luc2
    

    Use

    AAV and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-H2B-AID-EYFP

    Plasmid

    #47329

    Purpose

    for plasmid integration using the Flp-In system. AID at C-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    AID and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. As0

    Plasmid

    #123158

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR-ABS67, PhrpLE, PrinA

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJP-CoV2leader-nLuc-TSS

    Plasmid

    #175430

    Purpose

    Expresses nLuc with 5' leader of CoV2 in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' CoV2 leader
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJP-HBB-nLuc

    Plasmid

    #175431

    Purpose

    Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' HBB leader
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV7.1_3xFLAG-STK4

    Plasmid

    #172989

    Purpose

    constitutive expression of FLAG-tagged STK4

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-STK4 (STK4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDest27-mBIN1+13+17

    Plasmid

    #61097

    Purpose

    Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

    Depositor

    Robin Shaw

    Article

    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

    Insert

    mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pPEPZ-sgRNAclone

    Plasmid

    #141090

    Purpose

    This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

    Insert

    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p3

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. TERTpWT_Gluc_CCR5

    Plasmid

    #192270

    Purpose

    Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    Promoter Reporter Insert within donor template
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TERT promoter driving expression of gaussia

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST14-ARL3

    Plasmid

    #67376

    Purpose

    Expression of native hArl3 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL3 (ARL3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only