We narrowed to 17,913 results for: Ret;

1. pcDNA3-Venus-StagRFP-AktAR

   Plasmid

   #173020

   Purpose

   Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Venus-StagRFP-AktAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOZ-N-FH MERIT40

   Plasmid

   #27498

   Depositor

   Roger Greenberg

   Article

   Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

   Insert

   MERIT40 (BABAM1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pUAP4

   Plasmid

   #136079

   Purpose

   Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. G13 sensor (Ga13_2)

   Plasmid

   #112930

   Purpose

   A FRET sensor for G13 activation, encoded on a single plasmid.

   Depositor

   Dorus Gadella

   Article

   Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

   Insert

   G13 sensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOZ-N-FH KIAA0157

   Plasmid

   #27499

   Depositor

   Roger Greenberg

   Article

   Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

   Insert

   KIAA0157 (ABRAXAS2 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   March 1, 2011

   Availability

   Academic Institutions and Nonprofits only

6. L1_UBE2:mS-Lt-Ck3

   Plasmid

   #136131

   Purpose

   L1 in position 3, mark cell outline with mScarletI

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpUBE2:mScarletI-Lti6b_3tNos-35S
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pMSCV LDTS-eLIR

   Plasmid

   #189000

   Purpose

   lipophagy-inducing adaptor protein

   Depositor

   Atsushi Hoshino

   Article

   Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

   Insert

   LDTS-eLIR
   

   Use

   Retroviral

   Tags

   HA

   Available Since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

   Plasmid

   #115698

   Purpose

   ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

   Depositor

   Eckhard Boles

   Article

   Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

   Insert

   SSKar2-sfpHluorin-HDEL
   

   Tags

   ER retention signal containing HDEL and ER signal…

   Expression

   Yeast

   Mutation

   for introduced mutations see publication

   Promoter

   MET25

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Stx1a-12aa-mCer

   Plasmid

   #53236

   Purpose

   Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   Syntaxin 1A (Stx1a Rat)
   

   Tags

   12aa-linker and mCerulean

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

10. 3TERM_Nos-35S

    Plasmid

    #136116

    Purpose

    3TERM after CDS/ CDS12-CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    3TERM_Nos-35S (A. tumefaciens, CaMV)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pDJSVT86

    Plasmid

    #108965

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

    Type

    Empty backbone

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF-pIgH-ESCargo(FTV)

    Plasmid

    #140160

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Mito-Lapronic/pGW1

    Plasmid

    #140754

    Purpose

    FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

    Depositor

    Luis Felipe Barros

    Article

    Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

    Insert

    Mito-Lapronic
    

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCk3_spacer

    Plasmid

    #136073

    Purpose

    Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. PROM5_MpEF1a

    Plasmid

    #136113

    Purpose

    Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM5_MpEF1a Mapoly0024s0116
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Dimer cleaved

    Plasmid

    #198199

    Purpose

    FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mVenus-T2A-mTurquoise2
    

    Expression

    Mammalian

    Mutation

    A206K both

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. mKOkappa-2A-mTurquoise2

    Plasmid

    #98837

    Purpose

    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mKOkappa-2A-mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-EGFP-C1-HsAGO1_F

    Plasmid

    #146314

    Purpose

    Mammalian Expression of HsAGO1

    Depositor

    Elisa Izaurralde

    Article

    Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

    Insert

    HsAGO1 (AGO1 Human)
    

    Expression

    Mammalian

    Mutation

    N574S

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLS.491

    Plasmid

    #184956

    Purpose

    Test effect of a1/a2 length on editing rpoB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
    

    Expression

    Bacterial

    Mutation

    rpoB donor T1534C

    Promoter

    T7/lac

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

    Plasmid

    #140168

    Purpose

    Expresses a regulatable secretory cargo for Tetrahymena thermophila

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Other

    Promoter

    MTT1

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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